|
| Status |
Public on Sep 01, 2011 |
| Title |
The transcriptional modulator H2AFY marks Huntington's disease activity in men and mice |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To accelerate the development of disease-modifying therapeutics for Huntington’s disease (HD), a dynamic biomarker of disease activity and treatment response is critically needed.
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| |
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| Overall design |
Venous cellular whole blood from 8 patients with Huntington's disease (HD) and 6 control subjects were collected in PAXgene tubes (Wiagen, Valencia, CA). RNA was isolated from whole blood using the PAXgene blood RNA kit (Qiagene) with DNase treatment. The biotinylated complementary RNA was fragmented and hybridized to Affymetrix human genome U133A microarrays. The Affymetrix .CEL files were normalized to “all probe sets” in a standardized matter, and scaled to 100 by the MAS5 5.0 algorithm implemented in the Bioconductor package.
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| |
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| Contributor(s) |
Scherzer CR, Hu Y, Liao Z, Zheng B |
| Citation(s) |
21969577 |
| Submission date |
Sep 21, 2010 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Bin Zheng |
| Organization name |
Brigham and Women's hospital
|
| Department |
Center for Neurologic Diseases
|
| Street address |
65 Landsdowne Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (14)
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| Relations |
| BioProject |
PRJNA133053 |
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