|
| Status |
Public on Sep 20, 2011 |
| Title |
PA14_mexR vs. wildtype planktonic cells in minimal medium with C-30 |
| Platform organism |
Pseudomonas aeruginosa |
| Sample organism |
Pseudomonas aeruginosa PA14 |
| Experiment type |
Expression profiling by array
|
| Summary |
Mutations that made the cells insensitive to the QS inhibition by C-30 were identified in mexR, a multi-drug resistance operon repressor. Gene expression of mexR mutant relative to wild-type at the presence of C-30 was examined.
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| |
|
| Overall design |
Strains: PA14_mexR and wildtype.
Medium: OS minimal medium + 0.1% Adenosine as carbon source.
Compounds: 50 µM C-30 added at OD600=0.25.
Time: 2 hr.
Temp: 37 ºC.
Cell type: Planktonic Cells.
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| |
|
| Contributor(s) |
Maeda T, García-Contreras R, Pu M, Lee Y, Wood TK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 21, 2010 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Mingming Pu |
| E-mail(s) |
pu.mingming@gmail.com
|
| Organization name |
Texas A&M University
|
| Department |
Chemical Engineering
|
| Lab |
Thomas K. Wood
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (2) |
| GSM596626 |
PA14-WT QSM C30 2hr 37C planktonic cell |
| GSM596819 |
PA14-mexR QSM C30 2hr 37C planktonic cell |
|
| Relations |
| BioProject |
PRJNA132989 |
