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| Status |
Public on Sep 25, 2023 |
| Title |
Astrocyte growth during morphogenesis is driven by the Tre1/S1pr1 phospholipid-binding G protein-coupled receptor |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
TRAPseq was performed on Drosophila melanogaster astrocytes at different stages of development to assess the changes in gene translation that occur in astrocytes at these different stages.
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| Overall design |
Ribosomes were tagged in astrocytes using flies which expressed elaV-Gal80 and alrm-Gal4, UAS-EGFP-L10a. Ribsosomes were pulled down using GFP antibodies and the associated transcripts were isolated. Each sample contains 200 pooled fly heads; males and females were both used. Samples were isolated from flies at 4 timepoints: 48-72hr after puparium formation (P), 1-3 days post-eclosion (13), 10-12 days post-eclosion (1012) and 50-60 days post-eclosion (5060).
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| Contributor(s) |
Jay TR, Chen J, Freeman MR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 08, 2023 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Taylor Reagan Jay |
| E-mail(s) |
taylor.jay@case.edu, jayta@ohsu.edu
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| Organization name |
Oregon Health & Science University
|
| Department |
Vollum Institute
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| Lab |
Freeman Lab
|
| Street address |
3181 Sam Jackson Park Rd.
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (15)
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| GSM7767787 |
Pupal astrocytes (48-72hr APF)_biol rep1 |
| GSM7767788 |
Pupal astrocytes (48-72hr APF)_biol rep2 |
| GSM7767789 |
Pupal astrocytes (48-72hr APF)_biol rep3 |
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| Relations |
| BioProject |
PRJNA1014424 |
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