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| Status |
Public on May 22, 2024 |
| Title |
Effect of OGT inhibition on the epigenome of human LX-2 hepatic stellate cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Human LX-2 cells treated or not with OSMI-1 OGT inhibitor (OGTi) were used for H3K27ac ChIP-seq as well as for CoP-seq.
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| Overall design |
Human LX-2 cells were treated with vehicle (DMSO) or OSMI-1 OGT inhibitor (OGTi used at 50 µM for 24 h), cross-linked, chromatin was sonicated and used for H3K27ac ChIP (chromatin immunoprecipitation with Active Motif #39685 H3K27ac antibody) or CoP (Column Purified chromatin) assays followed by high-throughput sequencing.
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| Contributor(s) |
Very N, Boulet C, Eeckhoute J, Dubois-Chevalier J |
| Citation(s) |
38830870 |
| |
| Submission date |
Sep 13, 2023 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
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| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
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| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
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| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE243107 |
O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in hepatic stellate cells |
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| Relations |
| BioProject |
PRJNA1016369 |
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