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| Status |
Public on Sep 01, 2024 |
| Title |
The role of SDC4 in nucleus pulposus tissue homeostasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional analysis of 6-month-old primary nucleus pulposus (NP) mouse disc tissues from wild-type (WT) and SDC4 KO mice, which harbor deletions of exon 2 to part of exon 5 that endcodes the N-terminal coding region. Breeder mice were provided by Dr. Michael Simons.
SDC4's regulation of cytokine-dependent activation of the aggrecanse ADAMTS-5 is linked with inflammation-associated intervertebral disc degeneration. In this study, we aim to establish the role of SDC4 in matrix homeostasis under the context of aging.
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| Overall design |
Tissues from the nucleus pulposus (NP) were harvested from the intervertebral discs of four WT and four SDC4 KO mice at 6 months of age. NP and AF tissues were collected from lumbar discs in each animal for RNA extraction and hybridization using Affymetrix microarray.
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| Contributor(s) |
Sao K, Risbud MV |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 19, 2023 |
| Last update date |
Sep 01, 2024 |
| Contact name |
Makarand V Risbud |
| E-mail(s) |
makarand.risbud@jefferson.edu
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| Organization name |
Thomas Jefferson University
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| Department |
Orthopedic Surgery
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| Street address |
1025 Walnut St., Suite 511 College Building
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1018948 |
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