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| Status |
Public on Sep 25, 2010 |
| Title |
Global Changes following N-deprivation in Chlamydomonas: Illumina sequencing |
| Organism |
Chlamydomonas reinhardtii |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global analysis of transcripts under induced and non-induced conditions was applied as a first approach to study molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment. Towards this goal, high-throughput sequencing technology was employed to generate large numbers of expressed sequence tags of 8 biologically independent libraries (6 Illumina libraries, 2 454 libraries), four for each condition, N-replete and N-deprived, that allowed a statistically sound comparison of expression levels under the two tested conditions. Inferences on metabolism based on transcriptional analysis can only be indirect but were supported in parts by biochemical experiments. N-deprivation led to a marked redirection of metabolism as the carbon source acetate was no longer converted to cell building blocks by the glyoxylate cycle and gluconeogensis, but funneled directly into fatty acid biosynthesis. Protein biosynthesis and photosynthesis were down-regulated and genes of gametogenesis activated. A notable finding was that the genes encoding photosynthetic accessory PSBS proteins of currently unclear function in C. reinhardtii were strongly expressed following N-deprivation, providing a clue to their physiological role. Lipase-encoding genes were found to be some of the most regulated following N-deprivation, suggesting a remodeling of membranes under these conditions. The data provided here represent a rich source for the exploration of the mechanism of oil accumulation in microalgae.
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| Overall design |
Examining N-replete and N-deprived conditions. Three biological replicates each condition.
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| Contributor(s) |
Benning C |
| Citation(s) |
20935180 |
| |
| Submission date |
Sep 24, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Guangxi Wu |
| E-mail(s) |
wuguangx@msu.edu
|
| Organization name |
Michigan State University
|
| Street address |
S308 Plant Bio Bld
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9100 |
Illumina Genome Analyzer II (Chlamydomonas reinhardtii) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE24367 |
Global Changes following N-deprivation in Chlamydomonas |
|
| Relations |
| SRA |
SRP003630 |
| BioProject |
PRJNA133477 |
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