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Series GSE24365 Query DataSets for GSE24365
Status Public on Sep 25, 2010
Title Global Changes following N-deprivation in Chlamydomonas: Illumina sequencing
Organism Chlamydomonas reinhardtii
Experiment type Expression profiling by high throughput sequencing
Summary Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient-deprived. To begin studying the mechanisms underlying this process, N-deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global analysis of transcripts under induced and non-induced conditions was applied as a first approach to study molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment. Towards this goal, high-throughput sequencing technology was employed to generate large numbers of expressed sequence tags of 8 biologically independent libraries (6 Illumina libraries, 2 454 libraries), four for each condition, N-replete and N-deprived, that allowed a statistically sound comparison of expression levels under the two tested conditions. Inferences on metabolism based on transcriptional analysis can only be indirect but were supported in parts by biochemical experiments. N-deprivation led to a marked redirection of metabolism as the carbon source acetate was no longer converted to cell building blocks by the glyoxylate cycle and gluconeogensis, but funneled directly into fatty acid biosynthesis. Protein biosynthesis and photosynthesis were down-regulated and genes of gametogenesis activated. A notable finding was that the genes encoding photosynthetic accessory PSBS proteins of currently unclear function in C. reinhardtii were strongly expressed following N-deprivation, providing a clue to their physiological role. Lipase-encoding genes were found to be some of the most regulated following N-deprivation, suggesting a remodeling of membranes under these conditions. The data provided here represent a rich source for the exploration of the mechanism of oil accumulation in microalgae.
Overall design Examining N-replete and N-deprived conditions. Three biological replicates each condition.
Contributor(s) Benning C
Citation(s) 20935180
Submission date Sep 24, 2010
Last update date May 15, 2019
Contact name Guangxi Wu
Organization name Michigan State University
Street address S308 Plant Bio Bld
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL9100 Illumina Genome Analyzer II (Chlamydomonas reinhardtii)
Samples (6)
GSM600876 Chlamy_T_RNA_seq_1
GSM600877 Chlamy_T_RNA_seq_2
GSM600878 Chlamy_T_RNA_seq_3
This SubSeries is part of SuperSeries:
GSE24367 Global Changes following N-deprivation in Chlamydomonas
SRA SRP003630
BioProject PRJNA133477

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24365_RAW.tar 5.2 Gb (http)(custom) TAR (of TXT)
GSE24365_readme.txt 3.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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