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| Status |
Public on Sep 28, 2010 |
| Title |
Effect of enzyme IIANtr on gene expression in Escherichia coli |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
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| Summary |
Enzyme IIANtr (encoded by ptsN gene) is a component of the nitrogen phosphotransferase system (PTS). It has previously been shown that the dephosphorylated form of EIIANtr is required for the derepression of ilvBN encoding acetohydroxy acid synthase I (AHAS I) catalyzing the first step common to biosynthesis of branched-chain amino acids. Here we examine the effect of deletion of ptsN gene on global gene expression by microarray analysis. Most of the genes down-regulated in a ptsN mutant are controlled by sigma 70, while all the up-regulated genes are controlled by sigma S. As intracellular levels of sigma factors in the ptsN mutant were similar to those of the wild-type strain, this implied that the balance of sigma activities is modified by ptsN deletion.
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| Overall design |
The E. coli K12 strain MG1655 and its isogenic ptsN mutant were used in all experiments. Overnight bacterial cultures were incubated in M9 minimal medium containing 0.5% glucose as carbon source. Cells from duplicate experiments were harvested in exponential growth phase when cultures reached an A600 of 0.4. RNA was extracted from each sample using a QIAGEN RNA extraction kit according to the manufacturer's instruction.
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| Contributor(s) |
Lee C, Cho S, Kim H, Kim M, Peterkofsky A, Seok Y |
| Citation(s) |
21143318 |
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| Submission date |
Sep 27, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Yeong-Jae Seok |
| E-mail(s) |
yjseok@snu.ac.kr
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| Phone |
82-2-880-4414
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| Organization name |
Seoul National University
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| Department |
Biological Sciences
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| Street address |
San 56-1, Shillim-dong, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
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| Platforms (1) |
| GPL5482 |
Escherichia coli 5K oligo microarray |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA132971 |
