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Series GSE243982 Query DataSets for GSE243982
Status Public on May 06, 2024
Title Deciphering bat influenza H18N11 infection dynamics in Jamaican fruit bats on a single cell level
Organism Artibeus jamaicensis
Experiment type Expression profiling by high throughput sequencing
Summary Jamaican fruit bats (Artibeus jamaicensis) naturally harbor a wide range of viruses of human relevance. These infections are typically mild in bats, suggesting unique features of their immune system. To better understand the immune response to viral infections in bats, we infected Jamaican fruit bats with the bat-derived influenza A virus H18N11. Using comparative single-cell RNA sequencing, we generated a single-cell atlas of the Jamaican fruit bat intestine and mesentery, the target organs of infection. Gene expression profiling showed that H18N11 infection resulted in a moderate induction of interferon-stimulated genes and transcriptional activation of immune cells. H18N11 infection was prominent in various leukocytes, including macrophages, B cells, and NK/T cells. Confirming these findings, human leukocytes, particularly macrophages, were also susceptible to H18N11, highlighting the zoonotic potential of this virus. Our study provides insight into the virus-host relationship and thus serves as a fundamental resource for further characterization of bat immunology.
 
Overall design Jamaican fruit bats were infected with 5x10^5 TCID50 25 ┬ÁL of either WT H18N11 (n=6) or the NS1-deficient variant delNS1 (n=6). At 3 and 9 dpi 3 animals each were euthanized and the mesentery and intestine of each bat was processed for scRNA-seq. Four uninfected control bats were included.
 
Contributor(s) Andrieux G, Boerries M, Ciminski K, Kessler S, Schountz T
Citation(s) 38802391
Submission date Sep 25, 2023
Last update date Jun 12, 2024
Contact name Tony Schountz
E-mail(s) tony.schountz@colostate.edu
Organization name Colorado State University
Street address 3185 Rampart Rd
City Fort Collins
ZIP/Postal code 80523
Country USA
 
Platforms (1)
GPL33786 Illumina NovaSeq 6000 (Artibeus jamaicensis)
Samples (28)
GSM7802737 Mesentery, 3 dpi with WT H18N11, bat 1
GSM7802738 Mesentery, 3 dpi with WT H18N11, bat 3
GSM7802739 Mesentery, 9 dpi with WT H18N11, bat 1
Relations
BioProject PRJNA1020838

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Supplementary file Size Download File type/resource
GSE243982_RAW.tar 83.2 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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