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| Status |
Public on Feb 15, 2011 |
| Title |
Recruitment of BCL6 to target genes in Philadelphia chromosome positive acute lymphoblastic leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The Philadelphia chromosome (Ph) encodes the oncogenic BCR-ABL1 tyrosine kinase, which defines a subset of acute lymphoblastic leukemia (ALL) with a particularly unfavorable prognosis. Tyrosine kinase inhibitors (TKI) are widely used to treat patients with leukemia driven by BCR-ABL1 and other oncogenic tyrosine kinases. In response to TKI-treatment, BCR-ABL1 ALL cells upregulate BCL6 protein levels by ~90-fold, i.e. to similar levels as in diffuse large B cell lymphoma (DLBCL) with BCL6 translocations. In this study, we used genome tiling arrays to identify BCL6 target genes with specific recruitment of BCL6.
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| Overall design |
Three Ph+ ALL cell lines (BV-173, NALM-1 and TOM-1) in duplicate were either treated with 10µM STI571 (Imatinib) for 24 hours or cultured in absence of STI571.
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| Contributor(s) |
Duy C, Hurtz C, Shojaee S, Cerchietti L, Geng H, Swaminathan S, Klemm L, Kweon S, Nahar R, Braig M, Park E, Kim Y, Hofmann W, Herzog S, Jumaa H, Koeffler P, Yu J, Heisterkamp N, Graeber TG, Wu H, Ye H, Melnick A, Müschen M |
| Citation(s) |
21593872 |
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| Submission date |
Sep 28, 2010 |
| Last update date |
Sep 19, 2012 |
| Contact name |
Huimin Geng |
| E-mail(s) |
huimin.geng@ucsf.edu
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| Organization name |
UCSF
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| Department |
Department of Laboratory Medicine
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| Street address |
513 Parnassus Ave., MSB S-1480
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| GSM601447 |
BCL6 ChIP-chip in Tom1 cells - STI571 replicate 1 |
| GSM601448 |
BCL6 ChIP-chip in Tom1 cells + 10µM STI571 (24h) replicate 1 |
| GSM601449 |
BCL6 ChIP-chip in NALM1 cells - STI571 replicate 1 |
| GSM601450 |
BCL6 ChIP-chip in NALM1 cells + 10µM STI571 (24h) replicate 1 |
| GSM601451 |
BCL6 ChIP-chip in BV173 cells - STI571 replicate 1 |
| GSM601452 |
BCL6 ChIP-chip in BV173 cells + 10µM STI571 (24h) replicate 1 |
| GSM601453 |
BCL6 ChIP-chip in Tom1 cells - STI571 replicate 2 |
| GSM601454 |
BCL6 ChIP-chip in Tom1 cells + 10µM STI571 (24h) replicate 2 |
| GSM601455 |
BCL6 ChIP-chip in NALM1 cells - STI571 replicate 2 |
| GSM601456 |
BCL6 ChIP-chip in NALM1 cells + 10µM STI571 (24h) replicate 2 |
| GSM601457 |
BCL6 ChIP-chip in BV173 cells - STI571 replicate 2 |
| GSM601458 |
BCL6 ChIP-chip in BV173 cells + 10µM STI571 (24h) replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE24426 |
BCL6 enables leukemia cells to cope with inhibition of oncogenic tyrosine kinases |
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| Relations |
| BioProject |
PRJNA133747 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24404_RAW.tar |
148.1 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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