|
| Status |
Public on Jul 03, 2024 |
| Title |
CRISPR screen for regulators of cadherin based cell to cell adhesion |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Pooled Crispr based genome wide screen to find potential (transcriptional) regulators and pathways underlying the regulation of cadherin based cell to cell adhesion.
|
| |
|
| Overall design |
A spontaneously immortalized human keratinocyte line (HaCaT) expressing Cas9 was transduced with lentivirus containing the Brunello library encoding for 76441 sgRNAs inclusive 1000 non-targeting controls at a coverage of approximately 400 cells/sgRNA. The transduced cells were selected and expanded maintaining a library coverage of approximately 600 cells/sgRNA. 50-60x10^6 cells were stained Desmoglein 3 (Dsg3), and the 10 % cells with the lowest and the 10 % cells with the highest expression of Dsg3, respectively, were isolated by FACS. 50-60 x10^6 cells served as a control before FACS. Two technical replicates were done (repl 1 and 2).
|
| |
|
| Contributor(s) |
Stuedle C, Geier F, Spindler V, Franz H, Rathod M, Zimmermann A, Beyersdorfer V, Fischer KL |
| Citation(s) |
39271654 |
| |
| Submission date |
Oct 09, 2023 |
| Last update date |
Oct 07, 2024 |
| Contact name |
DBM Bioinformatics Core Facility |
| Phone |
+41612073541
|
| Organization name |
University of Basel
|
| Department |
Departement of Biomedicine
|
| Street address |
Hebelstrasse 20
|
| City |
Basel |
| State/province |
BS |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1026136 |
Less...
More...