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| Status |
Public on Jul 18, 2024 |
| Title |
Longitudinal Immune Profiling and Corticosteroid Modulation of the Immediate Innate Immune Response to ChAdOx1 nCoV-19 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChAdOx1 nCoV-19, a chimpanzee adenovirus (ChAd)-vector vaccine expressing the SARS-CoV-2 spike protein, has elicited robust immunological responses in large populations. Despite its proven efficacy and safety, some recipients have reported immediate inflammatory reactions post-vaccination. We comprehensively examined the immune landscape following ChAdOx1 nCoV-19 vaccination based on the epigenomic profiles of monocytes.
Glucocorticoids are wildly used as steroid anti-inflammatory drugs to modify acute and chronic inflammation. Recently, dexamethasone is used to modify inflammation in COVID19 and side-effects after COVID19 vaccination. ChAdox1 nCoV-19 induces interferon responses and inflammation in human. In addition to IFNa, TNF and IL1b treatment to mimic inflammatory environment gives general effects of glucocorticoids in complex inflammatory context.
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| Overall design |
Peripheral blood samples were obtained from five healthy volunteers at four post-ChAdOx1 nCoV-19 vaccination time points: days 0 (baseline), 1, 3, and 14.
To mimic COVID19 and inflammatory environment, we pre-treated combination of IFNa(1000U), TNF(20ng), and IL1b(20ug) to 1 million of PBMC derived CD14+ cells with 100mg/ml of MCSF. In order to investigate glucocorticoid effects in chromatin-remodeling level, we treated 100nM of Dexamethasone to each condition at 3h after cytokine treatment. Cells are havested 12h after dexamethasone treatment.
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| Contributor(s) |
Lee W, Park SH |
| Citation missing |
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| Submission date |
Oct 10, 2023 |
| Last update date |
Jul 19, 2024 |
| Contact name |
Wonhyo Lee |
| E-mail(s) |
wonhyo6446@unist.ac.kr
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| Organization name |
UNIST
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| Department |
Biological Sciences
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| Street address |
Unist-gil 50
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| City |
Ulju-gun |
| State/province |
Ulsan |
| ZIP/Postal code |
44919 |
| Country |
South Korea |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA1026530 |
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