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Status |
Public on Oct 10, 2024 |
Title |
Unraveling Epigenetic and Immune Mechanisms Underlying Cystogenesis in Autosomal Dominant Polycystic Kidney Disease [ChIP-Seq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a prevalent, life-threatening monogenetic disorder that affects millions worldwide. Currently, therapeutic options are limited and often associated with complications. To find more effective treatments, this study aimed to understand the molecular mechanisms driving cystogenesis in ADPKD. By analysing various epigenetic factors, we identified a set of master transcription factors that guided a core regulatory circuitry and remodeled chromatin accessibility in ADPKD. We further explored the role of immune cells, notably macrophages, in the disease’s progression using single-cell RNA sequencing. A subgroup of Lhfpl2+/Fat3+ macrophages, which reactivated the Hgf-Met-Nfkb1-Runx1 pathways, was found essential for cystic cell fate transition. Targeting the identified epigenetic regulators, H3K4me3 and TF Runx1, effectively halted disease progression in a mouse model. These findings may lead to innovative treatments for ADPKD in humans.
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Overall design |
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) in primary WT and PKD DBA positive cells.
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Contributor(s) |
Geng M |
Citation(s) |
- Liu Z, Zhang L, Chen Y. Epigenomic, cistromic, and transcriptomic profiling of primary kidney tubular cells. J Biol Methods 2024;11(2):e99010015. PMID: 39323486
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Submission date |
Oct 10, 2023 |
Last update date |
Oct 11, 2024 |
Contact name |
Jiayuan Chen |
E-mail(s) |
jy_chen@tmu.edu.cn
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Phone |
+8617320260315
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Organization name |
Tianjin Medical University
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Street address |
Heping
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City |
Tianjin |
State/province |
State... |
ZIP/Postal code |
300070 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (15)
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This SubSeries is part of SuperSeries: |
GSE244450 |
Unraveling Epigenetic and Immune Mechanisms Underlying Cystogenesis in Autosomal Dominant Polycystic Kidney Disease |
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Relations |
BioProject |
PRJNA1026595 |