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| Status |
Public on Oct 20, 2023 |
| Title |
Transcriptome Analysis Reveals Differentially Expressed Genes and Pathways for Oviduct Development and Defense in Prelaying and Laying Hens |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The oviduct plays an indispensable role in the formation of eggs, especially the magnum and uterus. The identification of oviduct development in different stages will help to target candidate genes and pathways in regulation of albumen and eggshell formation, as well as defense mechanism in oviduct and egg. To identify the function differences and the molecular defense mechanism of the oviduct and egg, we performed transcriptome sequencing analysis of the magnum and uterus in 120d and 300d old Lohmann layers, three birds in each group. With fold changes (log2 Ratio)>=2 and false discovery rate (FDR)<0.01, RNA-Seq revealed 1040 genes expressed differentially in the magnum and 595 genes in the uterus. By combining GO enrichment and KEGG pathway analysis, it served to show that genes activities of the magnum and uterus in prelaying chickens were more likely to concentrate on growth and development, and after egg-laying, they were mainly inclined to enhance the substances transmembrane transport and secretion activities. We further characterized 1579 new genes, while only 803 of them were functionally annotated. A complex mixture of proteins related to defense was measured in this study. A subset of avian β-defensins (AvBDs) and ovodefensins (OvoDs), that is, AvBD12, AvBD11, AvBD10, OvoDA1, OvoDB1, OvoDA2, OvoDA3 and OvoDBβ were detected to express in the magnum of laying hens at high levels. Collectively, the identification and functional analysis of these differentially expressed genes (DEGs), as well as specific expression of avian defensins may contribute to understand the development and defense mechanisms of oviduct and eggs.
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| Overall design |
To identify the function differences and the molecular defense mechanism of the oviduct and egg, we performed transcriptome sequencing analysis of the magnum and uterus in 120d and 300d old Lohmann layers.
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| Contributor(s) |
Yin L, Yu L, Zhang L, Ran J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 11, 2023 |
| Last update date |
Oct 20, 2023 |
| Contact name |
Lingqian Yin |
| Organization name |
Sichuan Agricultural University
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| Street address |
211 Huimin Road, Wenjiang, Chengdu, Sichuan Province
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| City |
Chengdu |
| ZIP/Postal code |
611120 |
| Country |
China |
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| Platforms (1) |
| GPL23499 |
Illumina HiSeq 4000 (Gallus gallus) |
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| Samples (12)
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| GSM7835922 |
300d Lohmann layers, magnum, rep1 |
| GSM7835923 |
300d Lohmann layers, magnum, rep2 |
| GSM7835924 |
300d Lohmann layers, magnum, rep3 |
| GSM7835925 |
120d Lohmann layers, uterus, rep1 |
| GSM7835926 |
120d Lohmann layers, uterus, rep2 |
| GSM7835927 |
120d Lohmann layers, uterus, rep3 |
| GSM7835928 |
300d Lohmann layers, uterus, rep1 |
| GSM7835929 |
300d Lohmann layers, uterus, rep2 |
| GSM7835930 |
300d Lohmann layers, uterus, rep3 |
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| Relations |
| BioProject |
PRJNA1026915 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE245083_All_gene_counts.txt.gz |
424.2 Kb |
(ftp)(http) |
TXT |
| GSE245083_All_gene_fpkm.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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