|
| Status |
Public on May 03, 2012 |
| Title |
Identification of genes induced on nitrate, role of OxyR |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
|
| Summary |
The study aimed to identify role of OxyR during growth on different electron acceptors when E. coli are growing anaerobically.
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| |
|
| Overall design |
Wt and OxyR null cells were grown on nitrate and fumarate medium anaerobically. Each condition was done in duplicate. RNA was extracted using Qiagen RNeasy kit after stabilization of RNA with RNA protect bacteria reagent. Hybridization and further processing was done based on Affymetrix protocols on E. coli Genome 2.0 arrays.
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| |
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| Contributor(s) |
Seth D |
| Citation(s) |
22539721 |
| |
| Submission date |
Oct 05, 2010 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Divya Seth |
| Organization name |
Case Western Reserve University
|
| Street address |
2106 Cornel Road
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
Ohio |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA132657 |
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