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| Status |
Public on Nov 30, 2023 |
| Title |
Characterization of regeneration initiating cells during Xenopus laevis tail regeneration [scRNA-Seq] |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this experiment, we revealed the critical steps for regeneration initiation. We discovered Regeneration Initiating Cells (RICs) using single cell and spatial transcriptomics of the regenerating Xenopus laevis tail. RICs are formed transiently from the basal epidermal cells and are critical for the modification of the surrounding extracellular matrix to allow for migration of other cell types that promote regeneration. Absence or deregulation of RICs leads to excessive extracellular matrix deposition and regeneration defects.
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| Overall design |
Single cell experiment was performed using 0, 1, 3, 12 hours post amputation (hpa) of Xenopus laevis tail at stage 40. Embryos were anesthetized and the regenerating part was dissected and collected. Tail pieces from 50 embryos were collected per one sample.
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| Contributor(s) |
Abaffy P, Zucha D, Naraine R, Kraus D, Sindelka R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 13, 2023 |
| Last update date |
Nov 30, 2023 |
| Contact name |
Radek Sindelka |
| E-mail(s) |
Radek.Sindelka@ibt.cas.cz
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| Organization name |
Institute of Biotechnology, Czech Academy of Science, v.v.i.
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| Lab |
Laboratory of Gene Expression
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| Street address |
Průmyslová 595
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| City |
Vestec by Prague |
| ZIP/Postal code |
25250 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL28901 |
Illumina NovaSeq 6000 (Xenopus laevis) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE245320 |
Characterization of regeneration initiating cells during Xenopus laevis tail regeneration |
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| Relations |
| BioProject |
PRJNA1027818 |
