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Series GSE24560 Query DataSets for GSE24560
Status Public on Jul 27, 2011
Title Comparative Expression Profiling of E. coli and S. aureus inoculated primary Mammary Gland Cells sampled from Cows with different genetic Predisposition for Somatic Cell Score
Organism Bos taurus
Experiment type Expression profiling by array
Summary Establishment of an in vitro system to explore molecular mechanisms of mastitis susceptibility in cattle by comparative expression profiling of Escherichia coli and Staphylococcus aureus inoculated primary cells sampled from cows with different genetic predisposition for somatic cell score
Primary bovine mammary gland epithelial cells (pbMEC) were sampled from the udder parenchyma of cows that were selected for high and low mastitis susceptibility by applying a marker assisted selection strategy considering QTL and molecular marker information of a repetitively confirmed QTL for SCS in the telomeric region on BTA18
The cells were cultivated and subsequently inoculated with heat inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus, respectively. After 1, 6 and 24 hours the cells were harvested and comparatively analyzed using microarray expression chip technology to identify differences in mRNA expression profiles attributed to cultivation, inoculation or to genetic predisposition.
 
Overall design Six heifers inheriting the favorable paternal QTL allele and five heifers inheriting the unfavorable QTL allele were selected by applying a marker assisted selection strategy. All heifers were kept under the same environmental conditions, had no clinical mastitis and did not show any indication of bacterial infection at slaughter. Primary bovine mammary gland epithelial cell cultures were established from cells sampled from the udder parenchyma of each cow. The cells were challenged with heat inactivated Escherichia coli and Staphylococcus aureus or with PBS for control. After 1, 6 and 24 hours the cells were harvested and mRNA expression was comparatively analyzed between time points for each treatment and each paternally inherited SCS-BTA18-QTL allele, respectively. In addition, the differences in gene expression at time points 1, 6 and 24h between inoculated and respective uninoculated control cells were investigated using the short time series expression miner STEM for co-expression profiling and GO cattegory enrichment analyses.
 
Contributor(s) Brand B, Schwerin M
Citation(s) 21702919
Submission date Oct 06, 2010
Last update date Dec 17, 2012
Contact name Bodo Brand
E-mail(s) brand@fbn-dummerstorf.de
Organization name Leibniz Institute of Farm Animal Biology
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
 
Platforms (1)
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (88)
GSM605490 pbMEC cultivated 1h, SCS-BTA18-Q-1
GSM605491 pbMEC cultivated 1h, SCS-BTA18-Q-2
GSM605492 pbMEC cultivated 1h, SCS-BTA18-Q-3
Relations
BioProject PRJNA132625

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24560_RAW.tar 180.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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