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| Status |
Public on Oct 07, 2010 |
| Title |
Expression profiling of cold-induced hybrid necrosis in triploid hybrids crossed between tetraploid wheat and Aegilops tauschii. |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
|
| Summary |
We have employed whole genome microarray expression profiling as a discovery platform to identify genes to alter the transcript accumulation levels in cold-induced hybrid necrosis lines, which are triploid hybrids crossed between tetraploid wheat and diploid wheat progenitor Aegilops tauschii. Of the up-regulated genes, defense-related genes were most frequently found in the leaves and shoot apical meristem (SAM) of type II necrosis line with low temperature. On the other hand, cell cycle-related genes were highly down-regulated in SAM of type II necrosis line under low temperature. To validate the microarray data, RT-PCR and quantitative RT-PCR analyses for 37selected genes were performed in total. Of the examined 21 up-regulated and 16 down-regulated genes, more than 75% of expression pattern were consistent with the microarray data.
Together with cytological analysis of the necrotic tissues, the microarray analysis strongly suggests that an autoimmune response-like reaction and repression of cell division might be triggered by intergenomic incompatibility between the AB and D genomes in type II necrosis.
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| Overall design |
Expression patterns were compared between the two synthetic hexaploid lines showing the wild-type phenotype (as a reference) and cold-induced hybrid necrosis. Total RNA samples were isolated from the 3-week-old seedling leaves. In addition, total RNA were also extracted from the seedling leaves and shoot meristem with 12- and 6-weeks of low temperature, respectively. Two independent experiments were conducted in each exprement.
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| Contributor(s) |
Mizuno N, Takumi S |
| Citation(s) |
21645146 |
| |
| Submission date |
Oct 06, 2010 |
| Last update date |
Jan 23, 2015 |
| Contact name |
Shigeo Takumi |
| Organization name |
Kobe University
|
| Department |
Graduate School of Agricultural Science
|
| Lab |
Plant Genetics
|
| Street address |
Rokkodai 1-1, Nada
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
657-8501 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL9805 |
Agilent-015520 Custom wheat 38k array |
|
| Samples (10)
|
| GSM605636 |
necrotic synthetic wheat-seedling leaf-rep1 |
| GSM605637 |
necrotic synthetic wheat-seedling leaf-rep2 |
| GSM605638 |
wild type synthetic wheat-seedling leaf with low temperature-rep1 |
| GSM605639 |
wild type synthetic wheat-seedling leaf with low temperature-rep2 |
| GSM605640 |
necrotic synthetic wheat-seedling leaf with low temperature-rep1 |
| GSM605641 |
necrotic synthetic wheat-seedling leaf with low temperature-rep2 |
| GSM605642 |
wild type synthetic wheat-seedling shoot meristem with low temperature-rep1 |
| GSM605643 |
wild type synthetic wheat-seedling shoot meri with low temperature-rep2 |
| GSM605644 |
necrotic synthetic wheat-seedling shoot meristem with low temperature-rep1 |
| GSM605645 |
necrotic synthetic wheat-seedling shoot meristem with low temperature-rep2 |
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| Relations |
| BioProject |
PRJNA132637 |
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