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| Status |
Public on Feb 21, 2024 |
| Title |
Two distinct waves of transcriptome and translatome remodelling drive germline stem cell differentiation |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Development and tissue maintenance require a supply of new cells, which are generated by division of stem cells and then specialised to perform their function. The differentiation process requires the tight control of gene expression, but this has been challenging to study in a systematic manner in adult stem cells, which are sparsely distributed in tissues. We have overcome these limitations by establishing a robust protocol to synchronise Drosophila female germline stem cell (GSC) differentiation in vivo, allowing us to perform transcriptome and translatome analyses at high temporal resolution during differentiation. As well as observing dynamic changes in mRNA level, promoter usage and exon inclusion, our data reveal that changing translation efficiency is the predominant regulatory mechanism during GSC differentiation. Indeed, changes in mRNA level are frequently buffered by changes in translation efficiency, including the translational repression of transcripts that will only be translated later during oocyte maturation or embryo development. Contrary to the expected linear accumulation of changes from stem cell to differentiated cell, our data reveal that differentiation occurs through two distinct waves of changes in both the transcriptome and translatome level.
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| Overall design |
Time course of RNAseq and Riboseq experiments in synchronised differentiating Drosophila germline stem cells in vivo.
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| Contributor(s) |
Samuels TJ, Gui J, Gebert D, Teixeira FK |
| Citation(s) |
38480936 |
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| Submission date |
Oct 27, 2023 |
| Last update date |
May 23, 2024 |
| Contact name |
Tamsin Joy Samuels |
| E-mail(s) |
tjs73@cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
Department of Genetics
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| Lab |
Felipe Karam Teixeira
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| Street address |
Downing Street
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3EH |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (28)
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| GSM7868240 |
RNA-seq bam,hs-bam 0.5hr AHS rep2 |
| GSM7868241 |
RNA-seq bam,hs-bam 5hr AHS rep1 |
| GSM7868242 |
RNA-seq bam,hs-bam 5hr AHS rep2 |
| GSM7868243 |
RNA-seq bam,hs-bam 9hr AHS rep1 |
| GSM7868244 |
RNA-seq bam,hs-bam 9hr AHS rep2 |
| GSM7868245 |
RNA-seq bam,hs-bam 18hr AHS rep1 |
| GSM7868246 |
RNA-seq bam,hs-bam 18hr AHS rep2 |
| GSM7868247 |
RNA-seq bam,hs-bam 28hr AHS rep1 |
| GSM7868248 |
RNA-seq bam,hs-bam 28hr AHS rep2 |
| GSM7868249 |
RNA-seq bam,hs-bam 38hr AHS rep1 |
| GSM7868250 |
RNA-seq bam,hs-bam 38hr AHS rep2 |
| GSM7868251 |
Ribo-seq bam,hs-bam no HS rep1 |
| GSM7868252 |
Ribo-seq bam,hs-bam no HS rep2 |
| GSM7868253 |
Ribo-seq bam,hs-bam 0.5hr AHS rep1 |
| GSM7868254 |
Ribo-seq bam,hs-bam 0.5hr AHS rep2 |
| GSM7868255 |
Ribo-seq bam,hs-bam 5hr AHS rep1 |
| GSM7868256 |
Ribo-seq bam,hs-bam 5hr AHS rep2 |
| GSM7868257 |
Ribo-seq bam,hs-bam 9hr AHS rep1 |
| GSM7868258 |
Ribo-seq bam,hs-bam 9hr AHS rep2 |
| GSM7868259 |
Ribo-seq bam,hs-bam 18hr AHS rep1 |
| GSM7868260 |
Ribo-seq bam,hs-bam 18hr AHS rep2 |
| GSM7868261 |
Ribo-seq bam,hs-bam 28hr AHS rep1 |
| GSM7868262 |
Ribo-seq bam,hs-bam 28hr AHS rep2 |
| GSM7868263 |
Ribo-seq bam,hs-bam 38hr AHS rep1 |
| GSM7868264 |
Ribo-seq bam,hs-bam 38hr AHS rep2 |
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| Relations |
| BioProject |
PRJNA1032864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246393_RAW.tar |
1.9 Gb |
(http)(custom) |
TAR (of BIGWIG, BW) |
| GSE246393_RNA_seq_fpkm.csv.gz |
1.4 Mb |
(ftp)(http) |
CSV |
| GSE246393_Ribo_seq_fpkm.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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