 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 27, 2023 |
| Title |
Hypermethylation leads to the loss of HOXA5, resulting in JAG1 expression and NOTCH signaling for renal fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
DNA methylation has been implicated in renal fibrosis. In this study, we demonstrated the induction of DNA methyltransferases and global DNA methylation in fibrotic kidneys, and further proved the beneficial effects of their inhibitor 5-Aza in renal fibrosis. HOXA5 was shown to be down-regulated in fibrotic kidneys through hypermethylation, and this down-regulation led to JAG1 expression and consequent activation of NOTCH signaling for renal fibrogenesis. These findings unveil DNA methylation and related genes, such as HOXA5, as potential therapeutic targets in renal fibrotic disease.
|
| |
|
| Overall design |
To investigate the differentially methylated genes in kidney fibrosis, C57BL/6 mice were subjected to unilateral ureter obstruction for 7 days (UUO7D with daily-saline injection), UUO 7D with daily-5'Aza injection, and sham operation. The genomic DNA from the kidney cortex and outer medulla were extracted for bisulfite sequencing.
|
| |
|
| Contributor(s) |
Xiao X, Shi H, Dong Z |
| Citation(s) |
38521405 |
| |
| Submission date |
Oct 27, 2023 |
| Last update date |
Mar 26, 2024 |
| Contact name |
Qingqing Wei |
| E-mail(s) |
QWEI@AUGUSTA.EDU
|
| Phone |
7067213551
|
| Organization name |
Augusta University
|
| Department |
Cellular Biology & Anatomy
|
| Lab |
CB1124
|
| Street address |
1460 Laney Walker Blvd.
|
| City |
AUGUSTA |
| State/province |
Georgia |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
| GSM7868587 |
mouse kidney, UUO7D, sample 1 [Saline1] |
| GSM7868588 |
mouse kidney, UUO7D, sample 2 [Saline2] |
| GSM7868589 |
mouse kidney, sham sample 1 for UUO7D [Sham] |
| GSM7868590 |
mouse kidney, sham for kidney ischemia/reperfusion [Sham48h] |
| GSM7868591 |
mouse kidney, UUO7D, 5AZA, sample 1 [5aza1] |
| GSM7868592 |
mouse kidney, UUO7D, 5AZA, sample 2 [5aza2] |
|
| Relations |
| BioProject |
PRJNA1032906 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246417_RAW.tar |
75.8 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...