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| Status |
Public on Apr 14, 2011 |
| Title |
DNA Methylation Dynamics in Human Induced Pluripotent Stem Cells over Time |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
We examined genome-wide DNA methylation with 22 human iPSC lines derived from different cell types and human ESC lines using Illumina’s Infinium HumanMethylation27 and focused on aberrant methylation sites in iPSCs for up to 42-week continuous cultivation. The iPSCs exhibited distinct epigenetic distances from ESCs at early passage. Continuous passaging of the iPSCs diminishes these differences between iPSCs and ESCs.
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| Overall design |
Bisulphite converted DNA from the 47 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
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| Contributor(s) |
Nishino K, Toyoda M, Yamazaki-Inoue M, Fukawatase Y, Chikazawa E, Sakaguchi H, Akutsu H, Umezawa A |
| Citation(s) |
21637780 |
| |
| Submission date |
Oct 13, 2010 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
umezawa@1985.jukuin.keio.ac.jp
|
| Phone |
81-3-5494-7047
|
| Organization name |
National Center for Child Health and Development
|
| Department |
Center for Regenerative Medicine
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (47)
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| Relations |
| BioProject |
PRJNA132363 |
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