|
| Status |
Public on Nov 04, 2024 |
| Title |
Effect of depletion of Ku70 on gene expression profiles of murine regulatory T cells in lung tumor microevnronment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The functions of the DNA repair protein Ku70 in immune homeostasis and tumor immunity remain unexplored. Here, we conducted deep sequencing of mRNA profiles from tumor-infiltrating Treg cells (from Foxp3Cre and Xrcc6fl/fl;Foxp3Cre mice injected with Lewis lung carcinoma cells). By comparing gene expression profiles, we found that Ku70-absent Treg displayed activated pathways in response to inflammatory factors and upregulated expression of genes negatively regulated by Foxp3. These findings strongly indicate that Ku70 plays a supportive role in enhancing Foxp3 transcriptional activity.
|
| |
|
| Overall design |
To investigate the role of Ku70 in the regulating of Treg function, we generated Treg cell-specific knockout mice (Xrcc6flox/floxFoxp3Yfp-Cre, cKO) and Foxp3Yfp-Cre (control, WT) mice. To establish lung metastasis model, LLC cells (1 million per mouse) were tail-intravenously injecting into 8- to 10-week-old WT and cKO mice. Tumors were excised at 21 days, and Tumor-infiltrating Treg cells were isolated for RNA sequencing.
|
| |
|
| Contributor(s) |
Huang Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 04, 2023 |
| Last update date |
Nov 04, 2024 |
| Contact name |
Qianru Huang |
| E-mail(s) |
hqr123@sjtu.edu.cn
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
227 South Chongqing Road Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1035787 |
Less...
More...