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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 25, 2024 |
| Title |
MERFISH reveals embryonic endothelial heterogeneity |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
The heterogeneity of embryonic endothelial cells (ECs) especially the distinction of arteriovenous ECs remains incompletely characterized. We established a mouse single-EC transcriptomic landscape at mid-to-late gestation stage and identified 19 subclusters, including Etv2+Bnip3+ early ECs and 2 specialized ECs. Most of these subtypes were grouped by their vascular-bed types, while ECs from brain, heart and liver were grouped by their tissue origins. Unlike arterial ECs (aECs), embryonic venous (vECs) and capillary ECs (cECs) shared less markers with their adult counterparts. Notably, capillary clusters showed some venous characteristics and one of them served as an intermediate state of arteriovenous specification. Compared to the more early stage, a clear arteriovenous branch which also going through a venous plexus was identified. aECs and vECs showed distinct transcriptional modules including specific regulatory networks of transcription factors. Especially, USF1 and MECOM were verified functioning in arteriovenous differentiation through human induced pluripotent stem cells (hiPSC) differentiation models. We therefore provide a new map of endothelial heterogeneity highlighting regulation of arteriovenous specification.
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| Overall design |
Vizgen MERSCOPE platform was employeed to perform the spatial transcriptome study. 2 sagittal sections were obtained from 2 different E9.5 wild-type embryos, and 1 sagittal section was obtained from wild-type E15.5 embryo. A 300-genes gene panel was used for probe hybridization.
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| Contributor(s) |
Chen J, Zhang X, Trembley M, Pu WT |
| Citation(s) |
38348657 |
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| Submission date |
Nov 09, 2023 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Xiaoran Zhang |
| E-mail(s) |
xiaoran.zhang@childrens.harvard.edu
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| Phone |
8574259855
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| Organization name |
Boston Children's Hospital
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| Department |
Cardiology
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| Lab |
Pu lab
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| Street address |
14 Perkins Square, Apt 11
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| City |
Jamaica Plain |
| State/province |
MA |
| ZIP/Postal code |
02130 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1038713 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE247450_RAW.tar |
48.3 Mb |
(http)(custom) |
TAR (of CSV) |
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