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Series GSE247474 Query DataSets for GSE247474
Status Public on Sep 08, 2024
Title Single-Cell Transcriptomic Analysis of Astrocytes 12 Hours after Ischemic Stroke.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Reactive astrocytes are detrimental or protective in ischemic stroke. However, the mechanisms of these effects are not entirely clear. We investigated the heterogeneity of mice astrocytes 12 h after cerebral ischemia-reperfusion via Single-cell RNA sequencing. We acquired astrocyte subclusters’ transcriptional characteristics involving diversified functions. We identified 9 genes as potential therapeutic targets and 6 genes were specific to astrocyte subcluster 2. Additionally, we explored the effects of melatonin and stattic on the transformation of astrocytes to the A2 phenotype. TTC stain, grip strength test and immunofluorescence were used. We revealed melatonin promoted the transformation of astrocytes to the A2 phenotype, the effect was related to STAT3 and inhibited by Stattic. Our study may lay a foundation for untangling the difference between astrocyte subclusters 12 h after stroke and elucidating the mechanisms of A2 astrocyte transformation 3 d after stroke.
 
Overall design Single-cell transcriptome analysis of two samples:one was a mixture of ischemic brain tissue from three MCAO model mice, the other was a mixture of brain tissue in the same area as the former from three sham mice.
 
Contributor(s) Wu Y, Gou X
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Submission date Nov 10, 2023
Last update date Sep 09, 2024
Contact name Yonghong Wu
Organization name Xi'an Medical University
Street address Xinwang Rd, Weiyang District
City Xi'an
ZIP/Postal code 710021
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM7890532 D2(SHAM)
GSM7890533 model-1(MCAO)
Relations
BioProject PRJNA1038750

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE247474_RAW.tar 55.5 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA

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