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| Status |
Public on Dec 15, 2023 |
| Title |
Extracellular miRNAs in serum and feces of mice exposed to high-dose radiation |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Exposure to high-dose radiation causes life-threatening serious intestinal damage. Histological analysis is the most accurate method for judging the extent of intestinal damage after death. However, it is difficult to predict the extent of intestinal damage to body samples. Here we focused on extracellular microRNAs (miRNAs) released from cells and investigated miRNA species that increased or decreased in serum and feces using a radiation-induced intestinal injury mouse model. A peak of small RNA of 25–200 nucleotides was detected in mouse serum and feces 72 h after radiation exposure, and miRNA presence in serum and feces was inferred. MiRNAs expressed in the small intestine and were increased by more than 2.0-fold in serum or feces following a 10 Gy radiation exposure were detected by microarray analysis and were 4 in serum and 19 in feces. In this study, miR-375-3p, detected in serum and feces, was identified as the strongest candidate for a high-dose radiation biomarker in serum and/or feces using a radiation-induced intestinal injury model.
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| Overall design |
In this study, miRNAs expression was examined in the small intestine, serum, and feces of mice exposed to high doses of radiation.
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| Contributor(s) |
Chiba M |
| Citation(s) |
38357239 |
| |
| Submission date |
Nov 15, 2023 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Mitsuru Chiba |
| E-mail(s) |
mchiba32@hirosaki-u.ac.jp
|
| Phone |
+81172395965
|
| Organization name |
Hirosaki University
|
| Department |
Graduate School of Health Sciences
|
| Lab |
Department of Bioscience and Laboratory Medicine
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| Street address |
66-1 Hon-cho
|
| City |
Hirosaki |
| State/province |
Aomori |
| ZIP/Postal code |
036-8564 |
| Country |
Japan |
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| Platforms (1) |
| GPL22383 |
Agilent-070155 Mouse miRNA Microarray (Feature Extraction Number version) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA1040859 |
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