|
| Status |
Public on May 01, 2024 |
| Title |
N2-alkyl-dG lesions trigger R-loop formation in genome |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Strand-specific kethoxal-assisted single-stranded DNA (ssDNA) sequencing (spKAS-seq) for mapping R-loop formation in HEK293T cells with/without the N2-heptynyl-dG treatment .
|
| |
|
| Overall design |
spKAS-seq was conducted following previously published procedures (PMID: 36449625) with minor modifications, where N2-heptynyl-dG was added to the culture medium until its final concentration reached 10 μM. After a 3-h incubation, the cells were treated with 5 mM N3-kethoxal dissolved in the culture medium for 10 min at 37°C, then harvested the genomic DNA for mapping the R-loop formation.
|
| |
|
| Contributor(s) |
Tang F, Wang Y |
| Citation(s) |
39351875 |
| |
| Submission date |
Nov 19, 2023 |
| Last update date |
Oct 09, 2024 |
| Contact name |
feng tang |
| E-mail(s) |
fengt@ucr.edu
|
| Phone |
9512411457
|
| Organization name |
University of California, riverside
|
| Department |
chemistry
|
| Lab |
Yinsheng Wang's lab
|
| Street address |
26861 Campus Point Dr., Moreno Valley
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92555 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1042825 |
Less...
More...