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| Status |
Public on Jun 10, 2011 |
| Title |
Small non-coding RNA expression in murine germ cells |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in a bovine serum albumin gradient. The small RNA fraction (18-36nt) was cloned and sequenced from total RNA of each cell type.
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| |
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| Overall design |
Three types of mouse germ cells small RNAs were used for high throughput sequencing analysis
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| |
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| Contributor(s) |
Gan H, Han C |
| Citation(s) |
21602304 |
| |
| Submission date |
Oct 20, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Chunsheng Han |
| E-mail(s) |
hancs@ioz.ac.cn
|
| Phone |
86-10-64807105
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Zoology
|
| Lab |
State Key Laboratory of Reproductive Biology
|
| Street address |
1-5 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (3) |
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| Relations |
| SRA |
SRP003871 |
| BioProject |
PRJNA131953 |
