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Status |
Public on Jun 10, 2011 |
Title |
Small non-coding RNA expression in murine germ cells |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in a bovine serum albumin gradient. The small RNA fraction (18-36nt) was cloned and sequenced from total RNA of each cell type.
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Overall design |
Three types of mouse germ cells small RNAs were used for high throughput sequencing analysis
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Contributor(s) |
Gan H, Han C |
Citation(s) |
21602304 |
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Submission date |
Oct 20, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Chunsheng Han |
E-mail(s) |
hancs@ioz.ac.cn
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Phone |
86-10-64807105
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Organization name |
Chinese Academy of Sciences
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Department |
Institute of Zoology
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Lab |
State Key Laboratory of Reproductive Biology
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Street address |
1-5 Beichen West Road, Chaoyang District
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City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
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Platforms (1) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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Samples (3) |
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Relations |
SRA |
SRP003871 |
BioProject |
PRJNA131953 |