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| Status |
Public on Sep 18, 2024 |
| Title |
Expression of genes involved in glycosylation pathway in mouse denrtic cells. |
| Platform organism |
Mus |
| Sample organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
These datasets uncover the regulation of glycosylation genes by TGF-beta in DC2.4 cells and dendritic cells (DCs) isolated from mice harboring MCA101 fibrosarcoma tumors.
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| Overall design |
Single cells suspensions from spleen and tumor (MCA101) were stained with antibodies directed against CD11c, MHC-II, CD103, CD8, F4/80, CD11b and CD45, and CD103-pos/CD8-pos DC1 cells purified using FACS sorting. DC2.4 cells were collected upon trypsinization after 4 days of 2.5 ng/ml TGF-beta stimulation. Expression of genes involved in glycosylation were quantified by quantitative real-time PCR (qRT-PCR) using the glycosylation RT2 Profiler PCR Array according to the manufacturer’s instructions (PAMM-046Z, Qiagen). Briefly, RNA was isolated using the RNeasy Kit (Qiagen), and 50-100 ng RNA was converted to cDNA using the RT2 First Strand Kit (Qiagen). The cDNAs were subjected to amplification using gene-specific primers and SYBR Green (RT2 SYBR Green Master Mix, Qiagen) in an Ab7100 Real-Time PCR System (Thermo Fisher Scientific).
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| Contributor(s) |
Sharma P, Zhang X, Ly K, Kim J, Wan Q, Kim J, Lou M, Kain L, Teyton L, Winau F |
| Citation(s) |
38207022 |
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| Submission date |
Nov 20, 2023 |
| Last update date |
Sep 19, 2024 |
| Contact name |
Pankaj Sharma |
| E-mail(s) |
Pankaj.sharma@childrens.harvard.edu
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| Organization name |
Boston Childrens Hospital
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| Street address |
220 Longwood Ave.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL33943 |
Qiagen RT2 glycosylation gene expression array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1043155 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248278_Fold_change.xlsx |
16.6 Kb |
(ftp)(http) |
XLSX |
| GSE248278_RAW.tar |
20.0 Kb |
(http)(custom) |
TAR (of TXT) |
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