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Series GSE248521 Query DataSets for GSE248521
Status Public on Nov 24, 2023
Title GPR15-mediated T cell recruitment during acute viral myocarditis facilitated virus elimination and improved outcome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Viral myocarditis is characterised by infiltration of mononuclear cells essential for virus elimination. GPR15 has been identified as a homing receptor for regulatory T cells in inflammatory intestine diseases, but its role in inflammatory heart diseases is still elusive. Therefore, we investigated the role of GPR15 during coxsackievirus B3‑induced myocarditis utilizing GPR15‑deficient mice as well as using in vitro experiments. Here we show that GPR15 deficiency had no effect on susceptibility to virus infection, but virus elimination was scant leading to adverse cardiac remodelling and impaired cardiac function. Delayed recruitment of regulatory T cells was accompanied by prolonged persistence of cytotoxic T cells as well as regulatory T cells in GPR15-deficient mice. Further, RNA sequencing revealed prolonged inflammatory response and altered chemotaxis in GPR15-deficient mice. In line, we identified GPR15 and its ligand GPR15L as an important chemokine receptor-ligand pair for the recruitment of regulatory and cytotoxic T cells. In summary, the insufficient virus elimination might be caused by a delayed recruitment of T cells as well as delayed interferon-γ expression, resulting in a prolonged inflammatory response and an adverse outcome in GPR15-deficient mice.
Overall design Heart tissue from GPR15 deficient and wildtype mice were investigated either with CVB3 induced myocarditis or controls
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Contributor(s) Lindner D
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Submission date Nov 23, 2023
Last update date Feb 23, 2024
Contact name Diana Lindner
Organization name University Hospital Freiburg
Department University Heart Center Freiburg-Bad Krozingen
Street address Hugstetter Straße 55
City Freiburg
ZIP/Postal code 79106
Country Germany
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (31)
GSM7916314 WT_sh_1
GSM7916315 WT_sh_2
GSM7916316 WT_sh_3
BioProject PRJNA1044422

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Supplementary file Size Download File type/resource
GSE248521_count_and_tpm.txt.gz 3.0 Mb (ftp)(http) TXT
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