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| Status |
Public on Dec 01, 2023 |
| Title |
Transciptome profile of TrmB and TbsP in Haloferax volcanii [RNA-seq] |
| Organism |
Haloferax volcanii DS2 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The hypersaline-adapted archaeon Haloferax volcanii exhibits remarkable plasticity in its morphology, biofilm formation, and motility in response to variations in nutrients and cell density. The transcriptional regulator TrmB maintains the rod shape in the related species Halobacterium salinarum by activating the expression genes involved in gluconeogenesis. Here we investigated the role of TrmB in Hfx. volcanii. The ∆trmB strain rapidly accumulated suppressor mutations in a gene encoding a novel transcriptional regulator, which we name TrmB suppressor, or TbsP. TbsP is required for adhesion to abiotic surfaces and maintains wild-type cell morphology and motility. To better understand the roles of TrmB and TbsP, we conducted expression profiling in ∆trmB, ∆tbsP, ∆trmB∆tbsP in response to glucose availability. TrmB and TbsP jointly regulate the transcription of gapII, fbp, and ppsA.
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| Overall design |
Transcripome profiling (RNA-seq) of TrmB and TbsP in the halophilic archaeon Haloferax volcanii in the presence and absense of glucose after 24 hours.
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| Contributor(s) |
Hackley RK, Bhanap P, Martinez Pastor M, Schmid AK |
| Citation(s) |
38204420 |
| |
| Submission date |
Nov 24, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Amy Schmid |
| E-mail(s) |
amy.schmid@duke.edu
|
| Organization name |
Duke University
|
| Street address |
3242 French Family Science Center
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL30888 |
Illumina NovaSeq 6000 (Haloferax volcanii DS2) |
| GPL33952 |
Illumina HiSeq 4000 (Haloferax volcanii DS2) |
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| Samples (39)
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| This SubSeries is part of SuperSeries: |
| GSE248566 |
Transciptome profile of TrmB and TbsP, Genome-wide binding of TbsP (HVO_2861) in Haloferax volcanii |
|
| Relations |
| BioProject |
PRJNA1044788 |
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