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| Status |
Public on Oct 22, 2010 |
| Title |
Seeds of Pisum sativum L. cv Alaska Early: non-aged controls vs. Aged |
| Organism |
Pisum sativum |
| Experiment type |
Expression profiling by array
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| Summary |
Seeds of Pisum sativum L. cv Alaska Early (purchased from the ‘Abundant Life Seed Foundation’, Port Townsend, USA) were equilibrated for approximately 5 weeks in tightly sealed boxes over 300 g-1 non-saturated LiCl (Hay et al. 2008), producing 60% relative humidity, in a temperature-controlled room (20 ± 1 ºC) until their water potential was stable at 12 % MC. Relative humidity was recorded with a Rotronic AWVC-D10 Hygropalm. These equilibrated seeds are referred to as 0 day ‘non-aged controls’. Following equilibration, accelerated ageing was carried out by placing seeds in sealed bottles at 50 °C until viability loss, thereafter referred to as ‘ageing’. Samples were taken at intervals after 8, 12, and 15 days of ageing. At each interval, germination tests were performed on 1 % water agar at 25 °C under warm white fluorescent light (15 micromol m-2 s-1) at a day / night cycle (8/16h). Germination was defined as radical emergence by at least 2 mm.
To summarize, we have performed a transcriptional profiling of Pisum sativum seeds comparing 0 day ‘non-aged controls’ with 8, 12 and 15 days of artificially aged seeds.
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| Overall design |
Four-condition experiment, 0 days, 8 days, 12 days and 15 days. Biological replicates: 3 for each comparison. Each biological replicate was independently grown and harvested.
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| Contributor(s) |
Osuna D, Chen H, Lorenzo O, Kranner I |
| Citation(s) |
24205239 |
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| Submission date |
Oct 21, 2010 |
| Last update date |
Nov 12, 2013 |
| Contact name |
Daniel Osuna |
| E-mail(s) |
daniel.osuna@usal.es
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| Phone |
34-923-294500
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| Fax |
34-923-294682
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| Organization name |
Centro Hispano-Luso de Investigaciones Agrarias.
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| Department |
Fisiología Vegetal, Universidad de Salamanca.
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| Lab |
7
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| Street address |
Río Duero, 12
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| City |
Villamayor |
| State/province |
Salamanca |
| ZIP/Postal code |
37185 |
| Country |
Spain |
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| Platforms (1) |
| GPL8288 |
UNIBI-Ps6kOLI1-Pisum sativum-16k-Microarray |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA132119 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24864_RAW.tar |
13.5 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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