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| Status |
Public on Jul 02, 2024 |
| Title |
Cut&Run analysis for transcription factors in MOLT-4 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Cut&Run analysis was performed in MOLT-4 cells to analyze the level of H3K27ac and DNA bindings of SMARCA4 and RUNX1 after DMSO or BRM014 treatment.
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| Overall design |
Treated MOLT-4 cells were incubated with CUTANA pA/G-Mnase. Chromtin fragments were purified utilizing a Cutana DNA purification kit. Libraries were generated using a NEBNext Ultra II DNA library prep kit and analyzed on Illumina NovaSeq at GENEWIZ Azenta Life Sciences Co. Ltd.
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| Contributor(s) |
Kim H, Tan SH, Tan TK, Sanda T |
| Citation(s) |
38969731 |
| |
| Submission date |
Dec 04, 2023 |
| Last update date |
Aug 11, 2024 |
| Contact name |
Tze King Tan |
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute
|
| Lab |
Takaomi Sanda Lab
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE230594 |
Oncogenic roles of SWI/SNF chromatin remodeling factors: therapeutic targets in T-cell acute lymphoblastic leukemia |
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| Relations |
| BioProject |
PRJNA1048514 |
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