NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE249983 Query DataSets for GSE249983
Status Public on Apr 02, 2024
Title Integrative analysis of the lncRNA-miRNA-mRNA Interactions in Smooth Muscle Cell Phenotypic Transitions II
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary To uncover new interactions between OCT4, lncRNA, and miRNA that may eventually help us in understanding not only OCT4-dependent effects, but also general causes of smooth muscle dysfunction associated with AS.
 
Overall design Primary mouse aortic smooth muscle cells (MASMC) were isolated and grown as previously described (Cherepanova et al., 2016). For experiments, cells at the 70% confluency were transfected with blocking siOct4 or non-target siRNA (NT siRNA) for 48 hours in serum-free media, followed by the treatment with 10 µg/mL POVPC (Cayman Chem, #10031) or vehicle (DMSO) for 24 hours based on our previous findings (Pidkovka et al., 2007). The samples were used for small RNA expression profiling using Arraystar Mouse small RNA Microarray (8x15K, Arraystar)
 
Contributor(s) Cherepanova OA, Mahajan A
Citation(s) 38660676
Submission date Dec 12, 2023
Last update date Apr 26, 2024
Contact name Olga Cherepanova
E-mail(s) cherepol@ccf.org, aatish.mahajan@gmail.com
Phone 216 387-5528
Organization name Cleveland Clinic Foundation
Department Cardiovascular and metabolic sciences
Street address 9620 Carnegie Avenue
City Cleveland
State/province Ohio
ZIP/Postal code 44195
Country USA
 
Platforms (1)
GPL31221 Arraystar Mouse SmallRNA expression V1 Microarray (086825)
Samples (12)
GSM7968436 Negative control non-target siRNA DMSO treated 1
GSM7968437 Negative control non-target siRNA DMSO treated 2
GSM7968438 Negative control non-target siRNA DMSO treated 3
Relations
BioProject PRJNA1051554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE249983_RAW.tar 8.1 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.