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Status |
Public on Apr 02, 2024 |
Title |
Integrative analysis of the lncRNA-miRNA-mRNA Interactions in Smooth Muscle Cell Phenotypic Transitions II |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
To uncover new interactions between OCT4, lncRNA, and miRNA that may eventually help us in understanding not only OCT4-dependent effects, but also general causes of smooth muscle dysfunction associated with AS.
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Overall design |
Primary mouse aortic smooth muscle cells (MASMC) were isolated and grown as previously described (Cherepanova et al., 2016). For experiments, cells at the 70% confluency were transfected with blocking siOct4 or non-target siRNA (NT siRNA) for 48 hours in serum-free media, followed by the treatment with 10 µg/mL POVPC (Cayman Chem, #10031) or vehicle (DMSO) for 24 hours based on our previous findings (Pidkovka et al., 2007). The samples were used for small RNA expression profiling using Arraystar Mouse small RNA Microarray (8x15K, Arraystar)
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Contributor(s) |
Cherepanova OA, Mahajan A |
Citation(s) |
38660676 |
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Submission date |
Dec 12, 2023 |
Last update date |
Apr 26, 2024 |
Contact name |
Olga Cherepanova |
E-mail(s) |
cherepol@ccf.org, aatish.mahajan@gmail.com
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Phone |
216 387-5528
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Organization name |
Cleveland Clinic Foundation
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Department |
Cardiovascular and metabolic sciences
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Street address |
9620 Carnegie Avenue
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City |
Cleveland |
State/province |
Ohio |
ZIP/Postal code |
44195 |
Country |
USA |
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Platforms (1) |
GPL31221 |
Arraystar Mouse SmallRNA expression V1 Microarray (086825) |
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Samples (12)
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GSM7968436 |
Negative control non-target siRNA DMSO treated 1 |
GSM7968437 |
Negative control non-target siRNA DMSO treated 2 |
GSM7968438 |
Negative control non-target siRNA DMSO treated 3 |
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Relations |
BioProject |
PRJNA1051554 |