 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 09, 2005 |
| Title |
Tiling microarray analysis of japonica and indica rice chromosome 10 |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
This experiment was designed to identify transcribed regions of both japonica and indica rice chromosome 10. A series of high-density oligonucleotide tiling arrays that represent sense and antisense strands of the entire nonrepetitive sequence of the chromosome were used to measure transcriptional activities. A total of 750,282 and 838,816 36mer oligonucleotide probes, positioned every 46 nt on average, were designed to interrogating the japonica and the indica chromosome, respectively. The probes were synthesized via maskless photolithography at a feature density of approximately 389,000 probes per slide. The arrays were hybridized with fluorescence-labeled cDNA reverse-transcribed from equal amounts of four selected poly(A)+ RNA populations, namely, seedling roots, seedling shoots, panicles, and suspension cultured cells of the respective rice subspecies.
Keywords: other
|
| |
|
| |
|
| Contributor(s) |
Li L, Wang X, Xia M, Stolc V, Su N, Peng Z, Li S, Tongprasit W, Wang J, Wang X, Deng X |
| Citation(s) |
15960804 |
| |
| Submission date |
Apr 06, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Xing-Wang Deng |
| E-mail(s) |
xingwang.deng@yale.edu
|
| Phone |
203-432-8908
|
| Organization name |
Yale University
|
| Department |
MCD Biology
|
| Street address |
165 Prospect
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL1933 |
Tiling microarray analysis of rice japonica chromosome 10(forward strand) |
| GPL1937 |
Tiling microarray analysis of rice indica chromosome 10 (forward strand) |
| GPL1938 |
Tiling microarray analysis of rice indica chromosome 10 (reverse strand) |
|
| Samples (6)
|
| GSM46493 |
Tiling microarray analysis of rice japonica chromosome 10(forward strand) |
| GSM46495 |
Tiling microarray analysis of rice japonica chromosome 10 reverse strand normal |
| GSM46497 |
Tiling microarray analysis of rice japonica chromosome 10 forward strand ,stressed |
| GSM46498 |
Tiling microarray analysis of rice japonica chromosome 10 reverse strand, stressed |
| GSM46637 |
Tiling microarray analysis of rice indica chromosome 10(forward strand, normal) |
| GSM46638 |
Tiling microarray analysis of rice indica chromosome 10(reverse strand, normal) |
|
| Relations |
| BioProject |
PRJNA91151 |
| Supplementary data files not provided |
|
|
|
|
 |
Less...
More...