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| Status |
Public on Feb 13, 2024 |
| Title |
CLIP-seq of Salmonella ProQ in intracellular-like conditions |
| Organism |
Salmonella enterica |
| Experiment type |
Other
|
| Summary |
The aim of the project is to identify transcriptome-wide binding sites for the global RNA-binding protein ProQ in Salmonella during intracellular-like conditions.
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| Overall design |
Salmonella enterica strain SL1344 carrying a chromosomal proQ-3xFLAG allele was grown in intracellular-like growth medium until and OD600 of 0.3 and thereafter subjected to crosslinking immunoprecipitation sequencing (CLIP-seq). Specifically, the bacteria were subjected to UV-light-induced crosslinking, anti-FLAG-mediated immunoprecipitation, RNase treatment, gel purification, and RNA purification, followed by Illumina library preparation using the NEBNext Small RNA Prep kit and sequencing on an Illumina NextSeq500 instrument at vertis Biotechnologie AG (Freising, Germany).
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| Contributor(s) |
Andresen L, Kjellin J, Holmqvist E |
| Citation(s) |
38411119 |
| Submission date |
Dec 12, 2023 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Erik Holmqvist |
| E-mail(s) |
erik.holmqvist@icm.uu.se
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| Organization name |
Uppsala University
|
| Street address |
Husargatan 3, Box 596, Uppsala University, Dept. of Cell and Molecular Biology
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| City |
Uppsala |
| State/province |
Uppland |
| ZIP/Postal code |
75124 |
| Country |
Sweden |
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| Platforms (1) |
| GPL27048 |
Illumina NextSeq 500 (Salmonella enterica) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1051606 |
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