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| Status |
Public on Dec 24, 2023 |
| Title |
Chromatin interactions of the EVI1 oncogene in myeloid leukaemia (ChIP-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
EVI1 expression is associated with poor prognosis in myeloid leukaemia. Aberrant expression can result from Chr.3q alterations, which cause juxtaposition of enhancers that induce EVI1 activation via long-range chromatin interactions. More often, however, EVI1 expression occurs unrelated to 3q alterations, and it is unclear if its expression underlies similar dependencies as 3q+ cells. As enhancers regulate promoters by physical interaction/chromatin looping, we explored if the EVI1 promoter in EVI1+3q- cells interacts with distally located chromatin and if these intereactions promote EVI1 expression. To monitor if these interactions involve active chromatin, we further performed H3K27ac-ChIP-Seq.
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| Overall design |
For this sample set, we performed ChIP-Seq using H3K27ac antibodies (abcam ab4729) as previously described (Boulianne et al, doi:10.1016/j.celrep.2017.01.057) using formaldehyde crosslinked DNA from CML patients' leukaemia cells.
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| Contributor(s) |
May PC, Robinson ME, Feldhahn N |
| Citation missing |
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| |
| Submission date |
Dec 13, 2023 |
| Last update date |
Dec 25, 2023 |
| Contact name |
Niklas Feldhahn |
| Organization name |
Imperial College London
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| Department |
Department of Medicine
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| Street address |
The Commonwealth Building, Hammersmith Hospital, Du Cane Road
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| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA1052086 |
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