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| Status |
Public on Jul 03, 2024 |
| Title |
Overexpression of miR-200s in murine ovarian cancer cells (ID8 and 28-2) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Little is known about the role of miR-200s in the progression of ovarian cancer. In this study we overexpressed the miR-200c/141 cluster, the miR-200b/200a/429 cluster, or both clusters in two different murine ovarian cancer cell lines (ID8 and 28-2). As co-oeverexpression of both clusters provided the best miR-200 overexpression, we evaluated ID8 and 28-2 cells overexpressing both miR-200 clusters (ID8-200f and 28-2-200f) compared to empty vector control cells (ID8EV and 28-2EV). ID8-200f and 28-2-200f cells showed increased expression of several mesenchymal genes, decreased proliferation, decreased invasion and increased apoptosis compared to their respective controls. RNA sequencing of ID8EV, ID8-200f, 28-2EV and 28-2-200f cells revealed that overexpression of miR-200s primary regulated genes involved in epithelial mesenchymal transition EMT) and gene implicated in migration. Therefore, our work suggests that miR-200s can inhibit characteristics associated ovarian cancer progression (increased proliferation and invasion and promotion of EMT).
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| Overall design |
Both the miR-200c/141 and the miR-200b/200a/429 clusters were overexpressed in ID8 and 28-2 cells creating ID8-200f and 28-2-200f cells. ID8EV and 28-2EV cells were also created by infecting with a emtpy lentiviral vector
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| Contributor(s) |
Moorehead R |
| Citation(s) |
39028700 |
| |
| Submission date |
Dec 14, 2023 |
| Last update date |
Aug 09, 2024 |
| Contact name |
Roger Moorehead |
| E-mail(s) |
rmoorehe@uoguelph.ca
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| Organization name |
University of Guelph
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| Department |
Biomedical Sciences
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| Street address |
50 Stone Rd E
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| City |
Guelph |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM7975387 |
ID8_control lentivirus and grown as 2D culture 1 |
| GSM7975388 |
ID8_control lentivirus and grown as 2D culture 2 |
| GSM7975389 |
ID8_control lentivirus and grown as 2D culture 3 |
| GSM7975390 |
ID8_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 1 |
| GSM7975391 |
ID8_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 2 |
| GSM7975392 |
ID8_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 3 |
| GSM7975393 |
28-2_control lentivirus and grown as 2D culture 1 |
| GSM7975394 |
28-2_control lentivirus and grown as 2D culture 2 |
| GSM7975395 |
28-2_control lentivirus and grown as 2D culture 3 |
| GSM7975396 |
28-2_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 1 |
| GSM7975397 |
28-2_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 2 |
| GSM7975398 |
28-2_lentivirus containing the miR-200c/141 cluster + lentivirus containing the miR-200b/200a/429 cluster_2D culture 3 |
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| Relations |
| BioProject |
PRJNA1052653 |
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