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Status |
Public on Oct 15, 2024 |
Title |
Single cell genomic analysis of PBMCs cells from a primary myelofibrosis patient |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
We performed single cell DNA sequencing by means of Tapestri platform on a mix of Peripheral Blood Mononuclear Cells (PBMCs) and CD34+ hematopoietic stem/progenitor cells (HSPCs) from a primary myelofibrosis (PMF). The main purpose of this project was to reconstruct the clonal composition of the neoplastic population by analyzing the mutational profile at the single-cell level.
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Overall design |
CD34+ cells were isolated through immunomagnetic selection from frozen PBMCs coming from a PMF patient. PBMCs and CD34+ cells were subjected to Tapestri analysis through a custom DNA panel.
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Contributor(s) |
Carretta C, Parenti S, Badii F, Bertesi M, Rontauroli S, Tavernari L, Genovese E, Sperduti S, Enzo E, Mirabile M, Pedrazzi F, Pessina C, Colugnat I, Mora B, Maccaferri M, Tenedini E, Martinelli S, Norfo R, Bianchi E, Casarini L, Potenza L, Luppi M, Tagliafico E, Guglielmelli P, Simoni M, Passamonti F, Vannucchi AM, Manfredini R |
Citation(s) |
39179669 |
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Submission date |
Dec 20, 2023 |
Last update date |
Oct 16, 2024 |
Contact name |
Rossella Manfredini |
E-mail(s) |
rossella.manfredini@unimore.it
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Organization name |
University of Modena and Reggio Emilia
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Street address |
Via Gottardi 100
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City |
Modena |
ZIP/Postal code |
41125 |
Country |
Italy |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (1) |
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Relations |
BioProject |
PRJNA1055070 |
Supplementary file |
Size |
Download |
File type/resource |
GSE251689_RAW.tar |
702.4 Mb |
(http)(custom) |
TAR (of LOOM, TAR) |
SRA Run Selector |
Raw data are available in SRA |
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