|
| Status |
Public on Apr 25, 2011 |
| Title |
Fetal Rat Testis mRNA Expression after 50 mg/kg Dibutyl Phthalate Exposure |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
High dose level dibutyl phthalate (DBP) exposure of fetal rat testes in vivo inhibits testosterone production (i.e. endocrine disruption). Here, fetal testis mRNA levels were profiled following exposure to a DBP dose level that did not significantly reduce testosterone levels. The goal was to identify the constellation of gene expression changes that do not correlate with endocrine disruption.
|
| |
|
| Overall design |
Fischer 344 rats were exposed via oral gavage of the dam to vehicle (corn oil) or 50 mg/kg (body weight) DBP daily from gestational day (GD) 12 to 20. The day after mating was defined as gestational day 0. Six hours after the final exposure on GD20, fetal testes were dissected and mRNA levels quantified using Affymetrix Rat Expression 230 2.0 microarrays.
|
| |
|
| Contributor(s) |
Johnson KJ, Xia Q |
| Citation(s) |
21266533 |
| Submission date |
Nov 08, 2010 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Kamin J Johnson |
| Organization name |
Alfred I. duPont Hospital for Children
|
| Department |
Nemours Biomedical Research
|
| Street address |
1600 Rockland Road
|
| City |
Wilmington |
| State/province |
DE |
| ZIP/Postal code |
19803 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA134305 |
Less...
More...