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Series GSE252092 Query DataSets for GSE252092
Status Public on Jun 12, 2024
Title The HMG-box module in FACT is critical for suppressing epigenetic variegation of heterochromatin in fission yeast
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary Chromatin condensation state is the key for retrieving genetic information. High-mobility group protein (HMG) proteins exhibit DNA-binding and bending activities, playing an important role in the regulation of chromatin structure. We have shown that nucleosomes tightly packaged into heterochromatin undergo considerable dynamic histone H2A-H2B maintenance via the direct interaction between HP1/Swi6 and facilitate chromatin transcription (FACT), which is composed of the Spt16/Pob3 heterodimer and Nhp6. In this study, we analyzed the role of Nhp6, an HMG box protein, in the FACT at heterochromatin. Pob3 mutant strains showed derepressed heterochromatin-dependent gene silencing, whereas Nhp6 mutant strains did not show significant defects in chromatin regulation or gene expression, suggesting that these two modules play different roles in chromatin regulation. We expressed a protein fusing Nhp6 to the C-terminus of Pob3, which mimics the multicellular FACT component Ssrp1. The chromatin-binding activity of FACT increased with the number of Nhp6 fused to Pob3, and the heterochromatin formation rate was promoted more strongly. Furthermore, we demonstrated that this promotion of heterochromatinization inhibited the heterochromatic variegation caused by epe1+ disruption. Heterochromatic variegation can be observed in a variety of regulatory steps; however, when it is caused by fluctuations in chromatin arrangement, it can be eliminated through the strong recruitment of the FACT complex.
 
Overall design Gene expression profile at exponentially-growing phase in the fission yeast deletion mutants of rhp6,pob3 and spt4.
 
Contributor(s) Takahata S, Taguchi A, Takenaka A, Mori M, Chikashige Y, Tsutsumi C, Hiraoka Y, Murakami Y
Citation(s) 38837646
Submission date Dec 27, 2023
Last update date Jun 12, 2024
Contact name Atsushi Matsuda
Organization name National Institute of Information and Communications Technology
Street address 588-2, Iwaoka, Iwaoka-cho, Nishi-ku
City Kobe
ZIP/Postal code 651-2492
Country Japan
 
Platforms (1)
GPL15360 Agilent-028936 KARC-EX-2010-31 026058 [ProbeName version]
Samples (9)
GSM7993570 S.pombe-wildtype-for-rep1
GSM7993571 S.pombe-wildtype-for-rep2
GSM7993572 S.pombe-wildtype-for-rep3
Relations
BioProject PRJNA1057767

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE252092_RAW.tar 23.6 Mb (http)(custom) TAR (of TXT)

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