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| Status |
Public on Jan 02, 2024 |
| Title |
Gene expression profiles in the inner ears of Fscn2 knockout mice compared with the control mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Fscn2-/- mouse is a typical model of age-related hearing loss which exhibits progressive hearing impairment and outer hair cell loss from 3 weeks of age. To investigate the molecular mechanism of hearing loss in Fscn2 knockout mice, gene expression profiling was performed on the inner ears for Fscn2+/+ and Fscn2-/- mice at 8 weeks of age using microarray analysis. Hearing screening tests at 8 weeks of age showed that the auditory brainstem response thresholds of Fscn2-/- mice were increased to 70-75 dB SPL at stimuli of click, while those of Fscn2+/+ mice were within the normal range(< 55 dB SPL). Microarray analysis identified a total of 244 differentially expressed mRNAs, including 72 upregulated and 172 downregulated genes, and a total of 688 differentially expressed lncRNAs, including 300 upregulated and 388 downregulated genes in Fscn2-/- mice, compared to Fscn2+/+ mice.
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| Overall design |
Gene expression profiling was performed on the inner ears for 3 Fscn2+/+ mice and 3 Fscn2-/- mice at 8 weeks of age using Agilent Mouse lncRNA Microrray V3. Hearing screening tests at 8 weeks of age showed that the auditory brainstem response thresholds of Fscn2-/- mice were increased to 70-75 dB SPL at stimuli of click, while those of Fscn2+/+ mice were within the normal range(< 55 dB SPL).
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| Contributor(s) |
Han F, Shang W, Ma Y, Zhang T |
| Citation missing |
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| Submission date |
Dec 28, 2023 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Wenjing Shang |
| E-mail(s) |
wenjshang@126.com
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| Organization name |
Binzhou Medical University
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| Street address |
NO.346 Guanhai Road
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| City |
Yantai |
| State/province |
Shandong |
| ZIP/Postal code |
364000 |
| Country |
China |
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| Platforms (1) |
| GPL25663 |
Agilent-084388 Mouse lncRNA Microrray V3 [Probe name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1058626 |
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