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Series GSE252240 Query DataSets for GSE252240
Status Public on Jan 31, 2024
Title Pig Taste Cell-Derived Organoids Synthesize Insulin
Organism Sus scrofa
Experiment type Expression profiling by high throughput sequencing
Summary Previously we showed that taste receptor cells in situ in taste buds synthesize insulin. Here we describe a model of pig taste organoid culture in which we have promoted insulin expression by induction of quiescence. The cellular heterogeneity of the lingual epithelium is maintained in the organoids, and stem cell type and organoid architecture can be controlled through changes in media composition and/or use of static versus dynamic culture. Pig taste organoids were maintained long term and organoids cultured in low sheer stress dynamic exhibited an architecture and expression profile akin to the native tissue. Porcine taste organoids also contained insulin, and the insulin critical transcription factors MAFA and PAX4. These results provide a pig model of taste organoid culture that can be used universally and bring us closer to the use of the taste tissue as a new renewable source of beta cells
 
Overall design In order to compare and contrast the gene expression profiles of porcine foliate organoids against the porcine foliate native tissue, total RNA was extracted from porcine foliate native tissue and the complementary organoid culture in Passage 1. This total RNA was then subjjected to RNA-Seq and differential gene expression analysis. Furthermore, to compare and contrast the gene expression profiles of porcine foliate organoids grown in LPM against porcine foliate organoids grown in LPM (day 0 to day 10) and then transfered to CQ (day 10 to day 16/17), total RNA was extracted (using Qiagen RNEasy MiniKit) from comparable organoid cultures grown only LPM, and cultures that were finished off with CQ. This total RNA was then subjected to RNA-Seq and differential gene expression analysis.
 
Contributor(s) Premathilake HU, Yao Q, Vegesna N, O'connell JF, Egan JM, Doyle ME
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Submission date Dec 29, 2023
Last update date Sep 30, 2024
Contact name Josephine M. Egan
E-mail(s) eganj@grc.nia.nih.gov
Organization name National Institute on Aging - NIH
Department Laboratory of Clinical Investigation
Lab Diabetes Section
Street address BRC Suite 100, 251 Bayview Boulevard
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
 
Platforms (1)
GPL26351 Illumina NovaSeq 6000 (Sus scrofa)
Samples (22)
GSM7997936 Foliate_native_tissue_Pig1
GSM7997937 Foliate_native_tissue_Pig2
GSM7997938 Foliate_native_tissue_Pig3
Relations
BioProject PRJNA1058996

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE252240_RAW.tar 60.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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