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Status |
Public on Jun 07, 2024 |
Title |
KAS-seq profiling captures transcription dynamics during oocyte maturation |
Organism |
Mus musculus |
Experiment type |
Other
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Summary |
In fully grown oocytes, the genome is considered to be globally transcriptionally silenced. However, this conclusion is primarily derived from the results obtained through immunofluorescence staining or inferred from the highly condensed state of chromosomes, lacking more direct evidence. Here, by using a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach, we investigated the landscape of single-strand DNA (ssDNA) throughout the genome and provided a readout of the activity and dynamics of transcription during oocyte meiotic maturation. In non-surrounded nucleolus (NSN) oocytes, we observed a robust KAS-seq signal, indicating the high transcriptional activity. In surrounded nucleolus (SN) oocytes, the presence of ssDNA still persists although the KAS-seq signal was relatively weak, suggesting the presence of transcription. Accompanying with the meiotic resumption, the transcriptional activity gradually decreased, and global repression was detected in matured oocytes. Moreover, we preformed the integrative genomics analysis to dissect the transcriptional dynamics during mouse oocyte maturation. In sum, the present study delineates the detailed transcriptional activity during mammalian oocyte maturation.
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Overall design |
We utilized the KAS-seq technique to construct libraries for various developmental stages of mouse oocytes (GV, GVBD, MII) to investigate the dynamic changes of ssDNA during oocytes maturation.
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Citation missing |
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Submission date |
Jan 02, 2024 |
Last update date |
Jun 07, 2024 |
Contact name |
huiqing an |
E-mail(s) |
hongzheng360@gmail.com
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Organization name |
Nanjing Medical University
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Street address |
101 Longmian Rd, Nanjing, Jiangsu, China
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City |
Nanjing |
ZIP/Postal code |
211166 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (16)
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Relations |
BioProject |
PRJNA1060267 |
Supplementary file |
Size |
Download |
File type/resource |
GSE252350_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of BW) |
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