|
| Status |
Public on Aug 21, 2011 |
| Title |
Nucleosome mapping data from GR and cell-cycle regulated genes in human cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Human UL3 cells (U20S derivatives containing GR and an MMTV-luc transgene) were treated with dexamethasone for 0, 1 or 4 hrs. Chromatin was harvested, digested with to ~70% mononucleosomes with MNase, and isolated mononucleosome fragments were used to probe custom Nimblegen genomic tiling microarrays.
In addition, human HL60 cells were treated with or without DMSO to induce granulocyte differentiaton, and mononucleosome positions mapped. MNase digested bare DNA fragments of ~500 bp average length from UL3 cells was used as a control.
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| |
|
| Overall design |
Mononucleosome positions at tiled genes for UL3 cells +/- dexamethasone and HL60 cells +/- DMSO.
|
| |
|
| Contributor(s) |
Schnitzler GR |
| Citation(s) |
21853138 |
| |
| Submission date |
Nov 11, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Gavin R. Schnitzler |
| E-mail(s) |
gavin.schnitzler@gmail.com
|
| Phone |
6086950428
|
| Organization name |
Tufts Medical Center
|
| Department |
Molecular Cardiology Research Institute
|
| Lab |
c/o Navin Kapur
|
| Street address |
800 Washington St. Box 80
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11196 |
Custom NimbleGen Nucleosome Position Analysis Array [080108_HG18_GS_ChIP] |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA134823 |
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