 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 01, 2024 |
| Title |
Human Airway and Alveolar Organoids from Bronchoalveolar Lavage Fluid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Many lung diseases remain understudied due to a lack of experimental models. Lung organoids, which consist of self-organizing epithelial cells, provide versatile in vitro models for normal and abnormal biology, drug screening, gene editing, and personalized therapeutics. However, human organoids are generally derived from lung tissue, which is not commonly obtained and represents only a small fraction of lung pathologies. Induced pluripotent stem cells have provided an important alternative but require complex manipulation. Recently, one study reported airway organoids from bronchoalveolar lavage (BAL) fluid, though sample sizes and characterization were limited. Here, we demonstrate robust establishment of airway organoids from a variety of human BAL samples and show that these organoids consist predominantly of basal cells plus differentiated airway cell types including secretory, ciliated, KRT13+ “hillock,” and ionocyte cells. Furthermore, we report the development of BAL-derived alveolar organoids comprised of alveolar type 2 (AT2) cells. These techniques significantly expand the scope of lung diseases that can be studied using safely accessible primary human cells.
|
| |
|
| Overall design |
Airway and alveolar organoids grown from human BAL fluid were analyzed by scRNA-seq and compared with organoids from human lung tissue.
***************************************************************
Submitter states that missing raw files are due to patient privacy concerns.
***************************************************************
|
| Web link |
https://www.atsjournals.org/doi/10.1164/rccm.202310-1831LE
|
| |
|
| Contributor(s) |
Liu M, Chen B, Borji M, Garcia de Alba Rivas C, Dost AF, Moye AL, Movval Abdulla N, Schnapp LM, Khalil HA, Wu CJ, Sharma NS, Kim CF |
| Citation(s) |
38652140 |
| |
| Submission date |
Jan 24, 2024 |
| Last update date |
Sep 20, 2024 |
| Contact name |
Carla Kim |
| E-mail(s) |
carla.kim@childrens.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1068661 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE254166_RAW.tar |
805.7 Mb |
(http)(custom) |
TAR (of H5, H5AD) |
| Raw data not provided for this record |
|
|
|
|
 |
Less...
More...