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Series GSE254180 Query DataSets for GSE254180
Status Public on Apr 01, 2024
Title Centromeric chromatin in mitosis reorganizes into a functional structure stabilized by cohesin [Halo-ChIP-seq]
Organism Gallus gallus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Three-dimensional organization of mitotic chromosomes is established by cohesin and condensin complexes. In the centromere, cohesin maintains sister chromatid pairing until anaphase onset, while condensin provides elastic resistance to spindle forces. However, how condensin and cohesin structure vertebrate centromeres remains unclear. By super-resolution imaging, Capture-C analysis and polymer modeling we show that vertebrate centromeres are partitioned into two condensin-dependent subdomains during mitosis. This bipartite sub-structure is found in human, mouse and chicken centromeres, and is therefore a fundamental feature of vertebrate centromere. Super-resolution imaging and electron tomography reveal that bipartite centromeres assemble bipartite kinetochores with each subdomain capable of binding a distinct microtubule bundle. Cohesin helps to link the centromere subdomains, limiting their separation in response to spindle forces and preventing merotelic attachments. The two-domain structure described here may have implications for avoiding chromosomal instability as uncoupling of centromere subdomains is a common feature of lagging chromosomes in cancer cells.
 
Overall design Caliburated Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for condensin I subnit (CAP-H) and condensin II subunit (CAP-H2) at, G2, prophase, and prometaphase.
 
Contributor(s) Deb M, Samejima K, Webb S, Earnshaw WC
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Submission date Jan 25, 2024
Last update date Apr 01, 2024
Contact name Shaun Michael Webb
E-mail(s) shaun.webb@ed.ac.uk
Organization name University of Edinburgh
Department Wellcome Trust Centre for Cell Biology
Lab Bioinformatics Core Facility
Street address 2.21 Swann Building, Kings Buildings
City Edinburgh
ZIP/Postal code EH9 3JR
Country United Kingdom
 
Platforms (1)
GPL32831 NextSeq 2000 (Gallus gallus)
Samples (32)
GSM8035585 WT_CDK1as_CAP-H-Halo_G2(biol rep1)_ChIP-Seq
GSM8035586 WT_CDK1as_CAP-H-Halo_G2(biol rep2)_ChIP-Seq
GSM8035587 WT_CDK1as_CAP-H-Halo_5min(biol rep1)_ChIP-Seq
This SubSeries is part of SuperSeries:
GSE254182 Centromeric chromatin in mitosis reorganizes into a functional structure stabilized by cohesin
Relations
BioProject PRJNA1068949

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Supplementary file Size Download File type/resource
GSE254180_RAW.tar 12.2 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
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