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| Status |
Public on Jun 26, 2024 |
| Title |
Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We discovered a role for the transcription factor OTX2 in formation of mouse definitive endoderm (DE). We determined the gene expression profiles during conversion of EpiSCs to DE using scRNA-seq. We used dTAG13 to deplete OTX2 at two timepoints of differentiation
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| Overall design |
Mouse EpiSCs were differentiated to DE with or without dTAG13 added at primitive streak or 24hr DE stage to deplete OTX2 for further 24hrs. Cells were harvested and live cells sorted by flow cytometry.
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| Contributor(s) |
Stadtfeld M, Ee L, Medina-Cano D |
| Citation(s) |
38854146 |
| |
| Submission date |
Jan 29, 2024 |
| Last update date |
Jun 27, 2024 |
| Contact name |
Ly-sha Ee |
| E-mail(s) |
lye4001@med.cornell.edu, worms917@yahoo.com
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| Organization name |
Weill Cornell Medicine
|
| Street address |
413 E 69th Street
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| City |
New York |
| ZIP/Postal code |
100021 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1070557 |
