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| Status |
Public on Dec 31, 2019 |
| Title |
Expression data from stratified mads-box (agl67) mutant and wild-type seeds, ABA-treated |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The mads-box mutant line showed an abscisic acid (ABA)-insensitive phenotype. The AGAMOUS-LIKE 67 (AT1G77950) gene encodes a transcription factor that is nuclear-localized, as observed by transiently transformed epidermal onion cells, specifically expressed in seeds and involved in an ABA signaling pathway during seed germination (Fernández-Arbaizar et al. 2012). SALK_050367 seeds were obtained from the Arabidopsis Biological Resource Center, ABRC.
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| Overall design |
To analyze AGAMOUS-LIKE 67 regulation of gene expression in a developmental time window of ABA responsiveness following breakage of seed dormancy (Lopez Molina et al. 2001, 2002), wild-type Col-0 and mads-box mutant seeds were stratified in water (4ºC, dark) during 3 days, and then transferred to light (22ºC, percival) in the presence of 5μM ABA for 3 hours. Three biological replicates were performed per seed type in order to get consistent results.
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| Contributor(s) |
Osuna D, Curto-Prieto M, Lorenzo O, Sanz L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 17, 2010 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Oscar Lorenzo |
| E-mail(s) |
oslo@usal.es
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| Phone |
34 923 294500 5117
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| Organization name |
Universidad de Salamanca
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| Department |
Dpt. Fisiologia Vegetal, Centro Hispano Luso de Investigaciones Agrarias (CIALE)
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| Lab |
lab. 7
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| Street address |
C/ Río Duero, 12 Campus de Villamayor. Parque Científico
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| City |
Salamanca |
| ZIP/Postal code |
37185 |
| Country |
Spain |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM629074 |
Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep1 |
| GSM629075 |
Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep2 |
| GSM629076 |
Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep3 |
| GSM629077 |
mads-box mutant seeds, T3h of 5μM ABA, biological rep1 |
| GSM629078 |
mads-box mutant seeds, T3h of 5μM ABA, biological rep2 |
| GSM629079 |
mads-box mutant seeds, T3h of 5μM ABA, biological rep3 |
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| Relations |
| BioProject |
PRJNA133815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25451_E1mads-box_vs_E1Col0.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE25451_Oliveros_JC_2007_FIESTA_VIEWER_STARTING_GUIDE.pdf.gz |
115.9 Kb |
(ftp)(http) |
PDF |
| GSE25451_RAW.tar |
11.8 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE25451_readme.txt |
728 b |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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