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Series GSE25451 Query DataSets for GSE25451
Status Public on Dec 31, 2019
Title Expression data from stratified mads-box (agl67) mutant and wild-type seeds, ABA-treated
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The mads-box mutant line showed an abscisic acid (ABA)-insensitive phenotype. The AGAMOUS-LIKE 67 (AT1G77950) gene encodes a transcription factor that is nuclear-localized, as observed by transiently transformed epidermal onion cells, specifically expressed in seeds and involved in an ABA signaling pathway during seed germination (Fernández-Arbaizar et al. 2012). SALK_050367 seeds were obtained from the Arabidopsis Biological Resource Center, ABRC.
Overall design To analyze AGAMOUS-LIKE 67 regulation of gene expression in a developmental time window of ABA responsiveness following breakage of seed dormancy (Lopez Molina et al. 2001, 2002), wild-type Col-0 and mads-box mutant seeds were stratified in water (4ºC, dark) during 3 days, and then transferred to light (22ºC, percival) in the presence of 5μM ABA for 3 hours. Three biological replicates were performed per seed type in order to get consistent results.
Contributor(s) Osuna D, Curto-Prieto M, Lorenzo O, Sanz L
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Submission date Nov 17, 2010
Last update date Dec 31, 2019
Contact name Oscar Lorenzo
Phone 34 923 294500 5117
Organization name Universidad de Salamanca
Department Dpt. Fisiologia Vegetal, Centro Hispano Luso de Investigaciones Agrarias (CIALE)
Lab lab. 7
Street address C/ Río Duero, 12 Campus de Villamayor. Parque Científico
City Salamanca
ZIP/Postal code 37185
Country Spain
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM629074 Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep1
GSM629075 Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep2
GSM629076 Wild-type Col-0 seeds, T3h of 5μM ABA, biological rep3
BioProject PRJNA133815

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25451_E1mads-box_vs_E1Col0.txt.gz 1.1 Mb (ftp)(http) TXT
GSE25451_Oliveros_JC_2007_FIESTA_VIEWER_STARTING_GUIDE.pdf.gz 115.9 Kb (ftp)(http) PDF
GSE25451_RAW.tar 11.8 Mb (http)(custom) TAR (of CEL)
GSE25451_readme.txt 728 b (ftp)(http) TXT
Processed data included within Sample table

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