|
| Status |
Public on Mar 19, 2024 |
| Title |
Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic KCl stress |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gene expression was quantified in S. cerevisae cells after 48 hours of treatment with KCl. crz1 and crz1 cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes that are independent of the downstream transcription factor Crz1. We find that calcineurin has very little effect on gene expression in response to KCl.
|
| |
|
| Overall design |
Gene expression was measured in cells lacking Crz1 (crz1) and cells lacking both Crz1 and calcineurin (crz1 cnb1). For each strain samples were collected before treatment and 48 hours following the addition of 400mM KCl. Triplicate experiments were performed.
|
| |
|
| Contributor(s) |
Flynn MJ, Li R, Zhu LJ, Benanti JA |
| Citation(s) |
38562881 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM136280 |
Molecular Mechanisms of Cell Cycle Control |
UNIV OF MASSACHUSETTS MED SCHOOL |
Jennifer A Benanti |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Jennifer Benanti |
| E-mail(s) |
Jennifer.Benanti@umassmed.edu
|
| Phone |
508-856-1773
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation St., LRB 525
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25927 |
BGISEQ-500 (Saccharomyces cerevisiae) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE254555 |
Identification of calcineurin-dependent gene expression changes in response to chronic stress |
|
| Relations |
| BioProject |
PRJNA1071034 |
Less...
More...