|
| Status |
Public on Feb 01, 2024 |
| Title |
Malassezia responds to environmental pH signals through the conserved Rim/Pal pathway |
| Organism |
Malassezia sympodialis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During mammalian colonization and infection, microorganisms must be able to rapidly sense and adapt to changing environmental conditions including alterations in extracellular pH. The fungus-specific Rim/Pal signaling pathway is one process that supports microbial adaptation to alkaline pH. This cascading series of interacting proteins terminates in the proteolytic activation of the highly conserved Rim101/PacC protein, a transcription factor that mediates microbial responses that favor survival in neutral/alkaline pH growth conditions, including many mammalian tissues. We identified the putative Rim pathway proteins Rim101 and Rra1 in the human skin colonizing fungus Malassezia sympodialis. Targeted mutation of these proteins confirmed their role in M. sympodialis growth at higher pH. Additionally, comparative transcriptional analysis of the mutant strains compared to wild-type suggested mechanisms for fungal adaptation to alkaline conditions. These signaling proteins are required for optimal growth in a murine model of atopic dermatitis, a pathological condition associated with increased skin pH. Together these data elucidate both conserved and phylum-specific features of microbial adaptation to extracellular stresses.
|
| |
|
| Overall design |
Comparing the transcriptome of three different strains of ATCC 42132 Malassezia sympodialis (WT, rim101∆, and rra1∆) in DMEM, pH 4, and pH 7.5 conditions. A total of 3 replicates were performed for each strain in each condition, for a total of 27 samples.
|
| |
|
| Contributor(s) |
Pianalto KM, Telzrow CL, Brown Harding H, Brooks JT, Gushiken-Ibañez E, LeibundGut-Landmann S, Heitman J, Ianiri G, Alspaugh JA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 30, 2024 |
| Last update date |
Feb 01, 2024 |
| Contact name |
J Andrew Alspaugh |
| E-mail(s) |
andrew.alspaugh@duke.edu
|
| Organization name |
Duke University
|
| Lab |
Alspaugh
|
| Street address |
337 Sands Research Building, 303 Research Drive, DUMC 102359
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL34153 |
Illumina HiSeq 4000 (Malassezia sympodialis) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA1071206 |
Less...
More...