 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 24, 2010 |
| Title |
2.5D tubulogenesis |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
|
| Summary |
Hepatocyte growth factor-induced three-dimensional tubulogenesis (3D) is a simple and highly controllable system for studying epithelial tubule initiation and maintenance. However, due to its limited efficiency and asynchronous development, isolating genes associated with specific morphological changes observed during tubule formation has been unfeasible. Here we report a significantly enhanced in vitro culture method called 2.5-dimensional tubulogenesis (2.5D). Detailed image analysis of 2.5D has revealed morphologically distinct stages -- monolayer, extension, and tubule -- and finds that these stages display in a highly synchronized manner. Using time-course transcriptional profiling, we collected genes whose expression changes specifically associate with extension and tubule stage.
|
| |
|
| Overall design |
Time-course microarrays to identify genes whose temporal regulation is associated with extension and tubule stages. Four biological replicates for each stage (monolayer, extension, and tubule).
|
| |
|
| Contributor(s) |
Kwon SH, Mostov KE |
| Citation(s) |
21236678 |
| |
| Submission date |
Nov 19, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
SH Kwon |
| Phone |
415-476-6047
|
| Fax |
415-514-0169
|
| Organization name |
University of California San Francisco
|
| Lab |
Mostov Lab
|
| Street address |
600 16th St./N214 Mostov lab
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11351 |
Agilent-015067 Canine 4x44K Microarray (Probe Name version) |
|
| Samples (12)
|
| GSM644342 |
Canine epithelial cells, monolayer stage, rep1 |
| GSM644343 |
Canine epithelial cells, extension stage, rep1 |
| GSM644344 |
Canine epithelial cells, tubule stage, rep1 |
| GSM644345 |
Canine epithelial cells, extension stage, rep2 |
| GSM644346 |
Canine epithelial cells, tubule stage, rep2 |
| GSM644347 |
Canine epithelial cells, monolayer stage, rep2 |
| GSM644348 |
Canine epithelial cells, extension stage, rep3 |
| GSM644349 |
Canine epithelial cells, tubule stage, rep3 |
| GSM644350 |
Canine epithelial cells, extension stage, rep4 |
| GSM644351 |
Canine epithelial cells, monolayer stage, rep3 |
| GSM644352 |
Canine epithelial cells, tubule stage, rep4 |
| GSM644353 |
Canine epithelial cells, monolayer stage, rep4 |
|
| Relations |
| BioProject |
PRJNA133765 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25514_RAW.tar |
84.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...